IRAK2 Rabbit Polyclonal Antibody for WB, ELISA - O43187

Core Product Specifications and Parameters

Biological Background: IRAK2 Function and Localization

• IRAK2 (Interleukin-1 receptor-associated kinase-like 2) is a cytoplasmic signaling protein involved in the IL-1 receptor pathway.
• Following IL-1 stimulation, IRAK2 associates with the IL-1 type I receptor and activates downstream pathways that promote transcriptional up-regulation and mRNA stabilization of target genes.
• IRAK2 mRNA and protein are detected in multiple tissues, including spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas, and peripheral blood leukocytes.
• The protein contains ATP-binding and nucleotide-binding motifs and is regulated by phosphorylation; its three-dimensional structure has been solved.
• The canonical isoform has a molecular weight of approximately 69 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–270 of human IRAK2, encompassing the N-terminal domain; this region may be conserved across species, consistent with reported cross-reactivity with mouse and rat.
• For Western blotting, consider using whole-cell lysates from IL-1-stimulated immune cells (e.g., PBMCs) or tissues expressing IRAK2 to enhance signal.
• In ELISA applications, the antibody can be used for direct or indirect detection of IRAK2; appropriate blocking and washing conditions should be optimized empirically.
• Because IRAK2 is a phosphoprotein, sample preparation with phosphatase inhibitors is recommended to preserve phosphorylation states potentially recognized by the antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with reliable access to raw materials for innate immunity and inflammation targets like IRAK2. Our portfolio includes validated polyclonal antibodies, monoclonal antibody pairs, and ancillary reagents suitable for immunoassay development. For niche targets and rare raw materials, CamelBio offers sourcing solutions to accelerate your assay development pipeline.