IPO11 Rabbit pAb for WB and ELISA - Q9UI26

Core Product Specifications and Parameters

Biological Background: IPO11 Function and Localization

• IPO11 encodes Importin-11, also known as Ran-binding protein 11 (RanBP11), a member of the importin beta family.
• Functions as a nuclear transport receptor, mediating nuclear protein import by recognizing nuclear localization signals (NLS) on cargo substrates.
• Facilitates docking of the importin-cargo complex to the nuclear pore complex (NPC) and its translocation through the pore in an energy- and Ran-dependent manner.
• Known imported cargoes include UBE2E3 and RPL12; substrate release occurs upon RanGTP binding on the nucleoplasmic side.
• Subcellular localization is primarily cytoplasmic and nuclear, shuttling between compartments as part of the import cycle.
• Post-translational modifications include acetylation and phosphorylation, which may modulate importin activity.
• Alternative splicing produces multiple isoforms, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment (aa 776-975); consider epitope accessibility in your application.
• For Western blotting, the predicted molecular weight is ~113 kDa; validate detection in your sample system using appropriate controls.
• For ELISA, optimize coating and detection conditions using recombinant IPO11 or relevant cell lysates.
• Cross-reactivity with human and mouse is reported; verify specificity in your experimental setting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers with one-stop access to IVD raw materials, including validated antibodies like this IPO11 polyclonal antibody for nuclear transport research. We offer antibody pairs, bulk reagents, and customized rare-target sourcing, complemented by technical consultation to advance your assay from concept to clinic.