IP3R Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q14643

Core Product Specifications and Parameters

Biological Background: IP3R Function and Localization

• Inositol 1,4,5-trisphosphate-gated calcium channel that, upon IP3 binding, mediates calcium release from the endoplasmic reticulum (PubMed:10620513; PubMed:27108797). Related references: PMID:10620513 PMID:27108797
• The channel undergoes conformational changes allowing transition from a closed to an open state, enabling calcium ion transfer across the ER membrane.
• Cytoplasmic calcium released via ITPR1 can trigger apoptosis through activation of the CAMK2 complex.
• ITPR1 forms a complex with HSPA9 and VDAC1, regulating mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER to the mitochondria.
• Regulates epithelial secretion of electrolytes and fluid through the interaction with AHCYL1.
• Modulates fertilization and egg activation by tuning calcium oscillation frequency and amplitude.
• Widely expressed; localizes to the endoplasmic reticulum membrane, cytoplasmic vesicle membranes, and the perinuclear region.
• Key post-translational modifications include phosphorylation, palmitoylation, and ubiquitination; also glycosylated with disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen targets a cytoplasmic region (aa 2200–2300); consider peptide competition assays to confirm binding specificity.
• For Western blotting, use a low percentage gel (e.g., 6-8%) and prolong transfer times due to the high molecular weight (~314 kDa).
• In immunofluorescence or IHC, optimize permeabilization conditions to access the ER-membrane protein (e.g., with Triton X-100 or saponin).
• Cross-reactivity with mouse and rat is reported; validate in your specific sample system and consider species-specific loading controls.
• For ELISA, coat plates with recombinant protein or immunizing peptide and establish linearity with known standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets like ITPR1/IP3R involved in calcium signaling and neurological disorders (e.g., spinocerebellar ataxia), we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development and procurement needs. Whether you require rare-target raw-material sourcing or large-scale production support, CamelBio is your reliable partner.