Integrin linked ILK Rabbit Monoclonal Antibody for WB, ELISA - Q13418

Core Product Specifications and Parameters

Biological Background: Integrin linked ILK Function and Localization

• Scaffold protein that mediates protein-protein interactions during focal adhesion assembly, cell adhesion, and migration. Related references: PMID:17420447, PMID:20005845, PMID:30367047
• Core component of the IPP (ILK-PINCH-PARVIN) complex, which binds F‑actin and promotes filament bundling required for force generation during cell spreading and migration. Related references: PMID:30367047
• Engages integrin‑β cytoplasmic tails, contributing to inside‑out integrin activation and adhesion; PARVA binding facilitates focal adhesion targeting. Related references: PMID:20005845
• Works with PARVG to regulate leukocyte adhesion and spreading. Related references: PMID:16517730
• Functions upstream of AKT1/PKB and GSK3, linking integrin signaling to cell survival and metabolism. Related references: PMID:9736715
• Required for mitotic spindle integrity by promoting AURKA‑mediated phosphorylation of TACC3. Related references: PMID:18283114
• Nuclear ILK associates with chromatin and may act as a negative regulator of transcription. Related references: PMID:17420447
• Localizes to focal adhesions, cell membrane, lamellipodia, cytoplasm, sarcomere, centrosome, and nucleus.
• Highly expressed in heart, skeletal muscle, pancreas, and kidney; weakly expressed in placenta, lung, and liver.
• Contains ankyrin repeat domains and a pseudokinase domain with ATP‑binding capability but no catalytic activity; classified as a scaffold protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 350–452 of human ILK (C‑terminal kinase‑like domain). The antibody detects a band at approximately 51 kDa in WB; consider using heart or skeletal muscle lysates as positive controls.
• Rabbit monoclonal format ensures high specificity and lot‑to‑lot reproducibility. Applications: WB, ELISA. For ELISA, optimize coating antigen and antibody titration.
• Cross‑reacts with human, mouse, and rat ILK. Validate in your species and sample system; slight molecular weight variations may occur due to post‑translational modifications or isoforms.
• No validated quantitative data or recommended working dilutions are provided; empirical optimization is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies like this anti‑ILK monoclonal antibody for integrin signaling and cell adhesion research. We supply optimized antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents, and can assist with sourcing rare‑target raw materials. From concept to clinic, our services support diagnostic development with reliable, research‑grade materials.