Integrin alpha V (ITGAV/CD51) Rabbit mAb for WB, IHC-P, FC (intra), ELISA - P06756

Core Product Specifications and Parameters

Biological Background: Integrin alpha-V Function and Localization

• Integrin alpha-V (ITGAV) is a cell surface receptor that heterodimerizes with various beta subunits (ITGB1, ITGB3, ITGB5, ITGB6, ITGB8) to form functional integrins.
• ITGAV-containing integrins recognize the RGD (Arg-Gly-Asp) motif present in extracellular matrix proteins such as vitronectin, fibronectin, fibrinogen, and laminin, mediating cell adhesion, migration, and signaling.
• ITGAV:ITGB3 binds to growth factors (FGF1, FGF2, IGF1, IGF2) and cytokines (NRG1, IL1B, fractalkine) to modulate critical signaling pathways including FGF, IGF, ERBB, IL1B, and CX3CR1-dependent signaling. Related references: PMID:18441324 PMID:28302677 PMID:19578119 PMID:28873464 PMID:20682778 PMID:29030430 PMID:23125415
• ITGAV:ITGB3 interacts with PLA2G2A via a distinct site, inducing conformational changes that enhance ligand binding. Related references: PMID:18635536 PMID:25398877
• ITGAV:ITGB3 and ITGAV:ITGB6 bind fibrillin-1 (FBN1) in an RGD-dependent manner, and ITGAV:ITGB6/ITGB8 activate TGF-beta-1 by releasing it from latency-associated peptide (LAP). Related references: PMID:12807887 PMID:17158881 PMID:15184403 PMID:22278742 PMID:28117447
• ITGAV is predominantly localized at the cell membrane and enriched at cell junctions and focal adhesions.
• ITGAV serves as a host cell receptor for several viruses, including Adenovirus, Coxsackievirus, Herpesvirus, Parechovirus, and West Nile virus, and is implicated in HIV-1 Tat-induced angiogenesis.
• Post-translational modifications include glycosylation, disulfide bond formation, and proteolytic cleavage, and ITGAV binds calcium and other metal ions essential for integrin function.

Experimental Guidance and Technical Tips

• The synthetic peptide immunogen corresponds to amino acids 949-1048 of human ITGAV, a region within the extracellular domain; this antigen design may be suitable for detecting both full-length and cleaved forms.
• For Western blot, the predicted molecular weight is ~116 kDa (full-length) or ~130 kDa (glycosylated); under reducing conditions, a heavy chain of ~125 kDa and a light chain of ~25 kDa may be observed after post-translational cleavage.
• For IHC-P, consider heat-induced antigen retrieval and verify fixation conditions; for flow cytometry (intracellular), permeabilization is required.
• The antibody cross-reacts with human, mouse, and rat; validate in the relevant sample system to confirm performance.
• These are general technical considerations; optimal experimental conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to validated IVD raw materials, technical services, and consulting support from concept to clinic. This integrin alpha-V monoclonal antibody is part of our extensive portfolio of cell adhesion and receptor research tools, backed by our capability to supply validated antibody pairs, optimized monoclonals, and bulk ancillary reagents. For rare or custom targets, CamelBio provides expert sourcing and development services to accelerate your diagnostic assay development.