Insulin Rabbit Polyclonal Antibody for WB, IHC-P, IF-P, ELISA - P01308

Core Product Specifications and Parameters

Biological Background: Insulin Function and Localization

• Insulin is a peptide hormone that decreases blood glucose concentration by increasing cell permeability to monosaccharides, amino acids, and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in the liver.
• It is secreted and acts as a key regulator of carbohydrate and glucose metabolism, with implications in diabetes mellitus.
• Insulin is specifically expressed by pancreatic beta-cells at the protein level.
• The protein undergoes proteolytic processing: cleavage on pair of basic residues generates the mature insulin A and B chains, which are linked by disulfide bonds.
• Alternative splicing produces multiple isoforms, and numerous disease variants are associated with diabetes and related disorders.
• Insulin is a critical pharmaceutical product for diabetes treatment, with its direct protein sequencing extensively studied.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–110 of human INS, covering the full preproinsulin sequence. Depending on the sample, the antibody may detect bands corresponding to proinsulin or mature insulin (~12 kDa) in WB. Validate molecular weight markers in your system.
• For IHC-P and IF-P, pancreatic tissue sections serve as an appropriate positive control. Consider antigen retrieval conditions and test appropriate antibody dilutions.
• In ELISA applications, this polyclonal antibody can be used as a capture or detection reagent. For sandwich ELISA development, pairing with a monoclonal anti-insulin antibody may improve specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive portfolio from validated antibody pairs to optimized monoclonal and polyclonal antibodies. For insulin and metabolic disease targets, we provide bulk ancillary reagents and specialize in sourcing rare-target raw materials to accelerate your diagnostic development. Trust CamelBio to be your seamless supply chain solution, from concept to clinic.