INSRR Rabbit Polyclonal Antibody for WB, ELISA - P14616

Core Product Specifications and Parameters

Biological Background: INSRR Function and Localization

• INSRR (Insulin receptor-related protein, IRR) belongs to the receptor tyrosine kinase family and shares homology with the insulin receptor.
• IRR functions as an extracellular pH sensor, activated by alkaline pH, and triggers intracellular signaling cascades involving IRS1 and AKT1/PKB.
• The protein is a single-pass transmembrane receptor localized to the cell membrane.
• Proteolytic cleavage on a pair of basic residues yields mature subunits that are linked by disulfide bonds.
• IRR undergoes glycosylation and phosphorylation, with key ATP- and nucleotide-binding domains essential for its kinase activity.
• The three-dimensional structure of the kinase domain has been solved, providing insights into its activation mechanism.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 747–921) maps to the cytoplasmic region of INSRR, which may detect both full-length and cleaved receptor forms.
• For Western blot, consider that the predicted molecular weight is approximately 144 kDa; however, cleavage can generate smaller subunits (e.g., α and β chains). Validate band patterns in the relevant sample system.
• The antibody cross-reacts with mouse and rat, expanding its utility to rodent models.
• For ELISA, the antibody can be used as a capture or detection reagent for quantifying INSRR levels in biological fluids or cell lysates; optimize pairing and concentrations experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibody reagents for emerging targets like pH-sensing receptors. This anti-INSRR polyclonal antibody supports development of immunoassays for insulin receptor-related signaling studies. We offer bulk sourcing, custom antibody development, and technical consultation to streamline your workflow from concept to clinic.