INPPL1 Rabbit Polyclonal Antibody for WB and ELISA - O15357

Core Product Specifications and Parameters

Biological Background: INPPL1 Function and Localization

• Enzymatic activity: INPPL1 functions as a phosphoinositide 5-phosphatase that dephosphorylates PtdIns(3,4,5)P3 to form PtdIns(3,4)P2, thereby negatively regulating the PI3K signaling pathway (PubMed:16824732). Related references: PMID:16824732
• Insulin signaling: It plays a central role in modulating insulin signaling by regulating PI3K-dependent pathways, although the detailed molecular mechanisms are still under investigation (PubMed:9660833). Related references: PMID:9660833
• Actin dynamics and migration: INPPL1 is critical for regulating the actin cytoskeleton, participating in membrane ruffling, cell adhesion, spreading, and HGF-induced lamellipodium formation (PubMed:11739414, PubMed:12676785, PubMed:15668240, PubMed:21624956, PubMed:12235291, PubMed:15735664). Related references: PMID:11739414, PMID:12676785, PMID:15668240, PMID:21624956, PMID:12235291, PMID:15735664
• EGFR and EGF signaling: It regulates EGFR internalization and degradation, and participates in EGF-mediated signaling by negatively modulating the PI3K-PKB pathway (PubMed:15668240, PubMed:11349134). Related references: PMID:15668240, PMID:11349134
• Immune cell function: INPPL1 acts as a negative regulator of FCGR2A and mediates inhibitory signaling from FCGR2B, highlighting its role in terminating signal transduction in immune cells (PubMed:12690104, PubMed:11016922). Related references: PMID:12690104, PMID:11016922
• Subcellular localization: The protein is found in the cytoplasm, cytoskeleton, cell projections (filopodia, lamellipodia), basal cell membrane, nucleus, and spindle poles, consistent with its roles in cytoskeletal remodeling and cell cycle regulation.
• Tissue specificity: INPPL1 is widely expressed, with highest levels in skeletal muscle, heart, and brain; it is also present in platelets and macrophages.
• Additional roles: It participates in endochondral ossification (PubMed:23273569) and is implicated in mitotic spindle orientation, ciliogenesis, and cell cycle homeostasis (By similarity). Related references: PMID:23273569

Experimental Guidance and Technical Tips

• Immunogen Design: The antibody was raised against a recombinant fragment spanning amino acids 100-310 of human INPPL1 (NP_001558.3). This region is located within the N-terminal domain and may confer reactivity with structurally conserved regions in mouse and rat orthologs.
• Western Blot: A band at approximately 139 kDa is expected; consider using a positive control such as HeLa or MCF7 whole-cell lysate, and validate detection in the species of interest.
• ELISA: The antibody can be used for direct ELISA against the immunogen; for sandwich ELISA applications, appropriate pairing with a detection antibody should be empirically validated.
• General: As a polyclonal antibody, lot-to-lot consistency should be verified for critical assays; optimization of dilution factors is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting support, streamlining the journey from concept to clinical application. This anti-INPPL1 (SHIP2) polyclonal antibody supports research into PI3K-dependent signaling, metabolic disease, cancer, and immune regulation, complementing our broad portfolio of validated antibodies and recombinant proteins. For bulk sourcing, customized raw-material requirements, or assay development assistance, our team is ready to help advance your projects.