iNOS Rabbit pAb - P35228

Core Product Specifications and Parameters

Biological Background: iNOS Function and Localization

• Produces nitric oxide (NO), a messenger molecule with diverse functions including mediating tumoricidal and bactericidal actions in macrophages. Related references: PMID:7504305 PMID:7531687 PMID:7544004
• Possesses nitrosylase activity, catalyzing S-nitrosylation of cytoplasmic targets like PTGS2/COX2. As part of the iNOS-S100A8/9 transnitrosylase complex, selectively S-nitrosylates GAPDH at Cys-247 and other targets including ANXA5, EZR, MSN, and VIM. Related references: PMID:25417112
• Enhances synthesis of pro-inflammatory mediators such as IL6 and IL8, contributing to inflammatory responses. Related references: PMID:19688109
• Localizes predominantly to the cytoplasm and cytosol.
• Expressed in liver, retina, bone cells, airway epithelial cells, and chondrocytes; not detected in platelets. Related references: PMID:7504305
• Contains heme, FAD, FMN, and NADP cofactors, and binds calmodulin, calcium, and zinc, functioning as a complex oxidoreductase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 948-1153 near the C-terminus; verify antibody specificity in your target system.
• For Western blot (WB), the predicted molecular weight is ~131 kDa; consider using a low-percentage gel and extended transfer times.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), include appropriate antigen retrieval and permeabilization steps, as iNOS is cytosolic.
• As a polyclonal antibody, lot-to-lot consistency may vary; validate each new lot with positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We supply validated antibody pairs, optimized polyclonal antibodies like this iNOS product, bulk ancillary reagents, and rare target raw materials tailored to your needs. Whether you are investigating nitric oxide signaling or inflammatory pathways, CamelBio streamlines your sourcing with comprehensive support.