INMT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O95050

Core Product Specifications and Parameters

Biological Background: INMT Function and Localization

• INMT (indolethylamine N-methyltransferase), also known as TEMT (thioether S-methyltransferase) or aromatic alkylamine N-methyltransferase, is a methyltransferase enzyme involved in metabolic detoxification and neurotransmitter metabolism.
• Functions as a thioether S-methyltransferase, active with a variety of thioethers, selenoethers, and telluroethers, playing a crucial role in the detoxification of selenium and related compounds.
• Catalyzes the N-methylation of tryptamine and structurally related compounds, potentially modulating indoleamine signaling.
• Localizes predominantly to the cytoplasm, as annotated in UniProt (SUBCELLULAR LOCATION).
• Widely expressed across human tissues, with highest levels in thyroid, adrenal gland, and lung; intermediate in heart, placenta, skeletal muscle, testis, and others; very low or absent in brain and peripheral blood leukocytes.
• Alternative splicing generates multiple isoforms, though their functional significance is not fully characterized.
• The canonical protein has a molecular weight of approximately 28.9 kDa (28891 Da) and belongs to the S-adenosyl-L-methionine-dependent methyltransferase family.

Experimental Guidance and Technical Tips

• For western blot (WB), expect a band near 29 kDa using lysates from high-expression tissues such as thyroid or lung; consider using validated positive controls.
• Immunogen covers full-length human INMT (amino acids 1-263), suggesting recognition of native and possibly denatured forms; assay conditions may require optimization for specific applications.
• In immunofluorescence/immunocytochemistry (IF/ICC), anticipate cytoplasmic staining; fixation and permeabilization protocols should be validated for the target cell type.
• The antibody cross-reacts with mouse INMT, enabling cross-species studies; however, sequence homology should be checked before applying to other species.
• For ELISA, pair with a capture antibody if developing a sandwich assay, and confirm reactivity with recombinant or native protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including this anti-INMT polyclonal antibody for methylation and detoxification research. With capabilities spanning validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, CamelBio supports procurement needs from target identification to assay development. Whether you require routine supplies or rare-target sourcing, our integrated platform bridges the gap from concept to clinic.