IL4 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P05112

Core Product Specifications and Parameters

Biological Background: IL4 Function and Localization

• IL4, also known as B-cell stimulatory factor 1 (BSF-1) and Binetrakin, is a secreted cytokine encoded by the IL4 gene on chromosome 5 in humans.
• It is secreted primarily by activated T-cells, mast cells, eosinophils, and basophils.
• IL4 plays a central role in regulating antibody production, hematopoiesis, and inflammation, particularly by inducing class II MHC expression on resting B-cells and enhancing IgE and IgG1 secretion.
  Related references: PMID:1993171
• IL4 also upregulates the low-affinity IgE receptor (CD23) on lymphocytes and monocytes, further modulating allergic and inflammatory responses.
  Related references: PMID:2521231
• Signaling occurs via two receptor complexes: type 1 (IL4R/IL2RG) on hematopoietic cells and type 2 (IL4R/IL13RA1) on nonhematopoietic cells, both leading to JAK-STAT pathway activation, primarily STAT6.
  Related references: PMID:10219247, PMID:11526337, PMID:18243101, PMID:7721895
• Beyond immunity, IL4 has roles in the central nervous system, including memory and learning (By similarity).
• The protein contains a signal peptide, disulfide bonds, and glycosylation sites, and is subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen fragment (aa 25-153) corresponds to the mature human IL4 protein and is designed to elicit reactivity against the native and recombinant cytokine.
• For Western blot, the theoretical molecular weight is ~17 kDa; however, glycosylation and disulfide bonding may result in a shifted band (15–20 kDa) under reducing conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), we recommend optimizing antigen retrieval and antibody dilution in the relevant tissue context.
• For ELISA, this polyclonal antibody can be evaluated as a capture or detection reagent paired with a matched monoclonal antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For immunology and cytokine research, we offer validated antibody pairs, recombinant proteins, and ancillary reagents. Contact us for bulk orders and rare target sourcing.