IL21R Rabbit pAb - Q9HBE5

Core Product Specifications and Parameters

Biological Background: IL21R Function and Localization

• Interleukin-21 receptor (IL21R), also known as CD360, NILR, or novel interleukin receptor, is encoded by the IL21R gene in humans.
• IL21R functions as a specific receptor for the cytokine interleukin-21 (IL-21), playing a crucial role in immune cell signaling and regulation.
• The protein is a single-pass type I membrane glycoprotein featuring a signal peptide, an extracellular ligand-binding domain, a transmembrane helix, and a cytoplasmic tail; it contains disulfide bonds and fibronectin type-III-like repeats typical of the class I cytokine receptor family.
• Subcellular localization is primarily at the plasma membrane, consistent with its role in ligand recognition and transmembrane signaling.
• Tissue expression is largely restricted to lymphoid organs, with highest levels detected in the thymus and spleen, reflecting its involvement in lymphocyte development and function.
• UniProt keywords highlight structural features such as signal, glycosylation, disulfide bonds, and transmembrane domains, as well as potential involvement in disease through chromosomal rearrangements (e.g., in some lymphomas).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20-240) of human IL21R, targeting the ligand-binding region; this design is suitable for detecting the receptor in native and denatured forms.
• For Western blotting, the predicted molecular weight of IL21R is approximately 59 kDa; use reducing SDS-PAGE conditions and validate with appropriate positive controls (e.g., lymphoid tissue lysates).
• In ELISA applications, the antibody can serve as a capture or detection reagent; pair with a compatible secondary antibody and optimize coating and blocking conditions for your specific assay format.
• Cross-reactivity with mouse IL21R has been noted; consider sequence alignment to assess species-specific epitope conservation when designing experiments.
• For quantitative or diagnostic use, titrate the antibody in the relevant sample matrix and confirm specificity with recombinant protein or knockout cell lines if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with one-stop access to premium IVD raw materials, encompassing validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For IL21R-related immunoassay development, our portfolio supports cytokine receptor research and immune monitoring applications. With comprehensive supply capabilities from concept to clinic, CamelBio streamlines your raw-material procurement and accelerates assay development.