IL17A Rabbit Polyclonal Antibody - Q16552

Core Product Specifications and Parameters

Biological Background: Interleukin-17A Function and Localization

• Interleukin-17A (IL-17A) is a pro-inflammatory cytokine and the founding member of the IL-17 family, with a molecular weight of ~17.5–18 kDa.
• It functions as a key effector cytokine in antimicrobial host defense and maintenance of tissue integrity, bridging adaptive and innate immunity (PubMed:24120361). Related references: PMID:24120361
• IL-17A signals through the IL17RA/IL17RC receptor complex, activating TRAF6-dependent NF-κB and MAP kinase pathways to drive transcription of inflammatory mediators (PubMed:17911633, PubMed:18684971, PubMed:19825828). Related references: PMID:17911633 PMID:18684971 PMID:19825828
• As the hallmark cytokine of Th17 cells, IL-17A promotes neutrophil chemotaxis and activation via induction of chemokines such as CXCL1 and CXCL5 in the airway epithelium (By similarity).
• It contributes to epithelial barrier integrity by regulating tight junction formation (e.g., OCLN localization) and by synergizing with IL-17F to induce antimicrobial β-defensins (PubMed:21350122). Related references: PMID:21350122
• IL-17A is secreted and expressed in memory Th17 cells, and it participates in germinal center formation and antiviral responses (By similarity).
• Post-translational features include glycosylation and conserved disulfide bonds, important for its dimeric structure and stability (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature form of IL-17A (aa 24–155), covering the active cytokine domain; consider validating antibody specificity in relevant human, mouse, or rat samples due to the reported cross-reactivity.
• For Western blot, a band near 18 kDa is expected; glycosylation may cause slight molecular weight shifts.
• This polyclonal antibody is suitable for ELISA and may be considered for cytokine quantification assays, though optimal dilution and buffer conditions should be determined empirically.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), appropriate antigen retrieval methods and antibody titration are recommended to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This IL-17A polyclonal antibody supports investigations into Th17 biology, inflammatory diseases, and host-pathogen interactions. In addition to validated monoclonal/polyclonal antibodies, CamelBio offers customized sourcing of antibody pairs, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development.