IL17A Rabbit Monoclonal Antibody for IHC-P and ELISA - Q16552

Core Product Specifications and Parameters

Biological Background: IL17A Function and Localization

• Interleukin-17A (IL17A), also known as CTLA-8, is a secreted effector cytokine of the innate and adaptive immune systems, encoded by the IL17A gene in humans.
• It signals through the heterodimeric receptor IL17RA-IL17RC, recruiting TRAF3IP2 and activating NF-kappa-B and MAP kinase pathways to induce transcription of cytokines, chemokines, and antimicrobial peptides. Related references: PMID:17911633, PMID:18684971, PMID:19825828
• IL17A is the signature cytokine of T-helper 17 (Th17) cells and primarily promotes neutrophil activation and recruitment to sites of infection and inflammation (By similarity).
• It plays a crucial role in antimicrobial host defense against extracellular bacteria and fungi by enhancing neutrophil responses and inducing antimicrobial beta-defensins in mucosal epithelial cells, often in synergy with IL17F. Related references: PMID:24120361, PMID:21350122
• IL17A contributes to the maintenance of epithelial barrier integrity by regulating tight junction proteins such as OCLN, and supports mucosal immunity induced by commensal bacteria. Related references: PMID:21350122
• It is involved in antiviral defense, enhancing T cell cytotoxicity against West Nile virus and promoting B cell responses in influenza infection (By similarity).
• Subcellularly, IL17A is secreted and expressed at the protein level in memory Th17 cells; it contains disulfide bonds and is glycosylated (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature form (aa 24-155) of human IL17A, suggesting the antibody likely recognizes the secreted cytokine. Consider validating reactivity with the native protein in relevant human samples.
• For IHC-P, optimize antigen retrieval conditions and include appropriate controls using tissues known to express Th17 cells, such as inflamed skin or gut mucosa.
• In ELISA, titrate the antibody as a capture or detection reagent and confirm specificity with recombinant human IL17A protein; cross-reactivity with other IL17 family members can be assessed if needed.
• Since the antibody is monoclonal, expect consistent lot-to-lot performance; verify the working dilution in your own experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this IL17A Rabbit mAb, which targets a key inflammatory cytokine. We support the entire development workflow with bulk ancillary reagents and rare target sourcing capabilities to accelerate your immunoassay projects in the immune response and inflammation research area.