IL13 Rabbit pAb - P35225

Core Product Specifications and Parameters

Biological Background: Interleukin-13 Function and Localization

• Interleukin-13 (IL-13), also known as P600, is a secreted cytokine encoded by the IL13 gene in humans, with a molecular weight of approximately 16 kDa (UniProt: P35225).
• IL-13 plays a critical role in allergic inflammation and immune responses to parasite infections, synergizing with IL-2 to regulate interferon-gamma synthesis. Related references: PMID:8096327 PMID:8097324
• It stimulates B-cell proliferation and activates eosinophils, basophils, and mast cells, which are key effector cells in allergic reactions. Related references: PMID:7903680 PMID:8759755
• IL-13 exerts its effects through a receptor complex of IL4R and IL13RA1, activating JAK1/TYK2 and STAT6, while IL13RA2 acts as a decoy receptor mediating internalization and depletion of IL-13. Related references: PMID:9013879 PMID:21622864
• The cytokine downregulates proinflammatory responses by suppressing NF-kappa-B and reducing synthesis of IL1, IL6, IL10, IL12, and TNF.
• IL-13 induces expression of vascular cell adhesion protein 1 (VCAM1) on endothelial cells, facilitating eosinophil recruitment. Related references: PMID:8639787
• In the esophageal epithelium, IL-13 triggers an inflammatory response that disrupts desmosome junctions by downregulating DSG1. Related references: PMID:24220297
• Post-translational modifications include disulfide bonds and glycosylation, and the protein is secreted into the extracellular space.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature IL-13 sequence (aa 25-146), lacking the signal peptide, so the antibody may preferentially recognize the secreted form.
• For Western blot, expect a band near 16 kDa; reducing conditions may improve detection.
• Reported cross-reactivity with mouse suggests potential utility across species, but validation in the specific experimental system is advisable.
• For IHC-P, optimize antigen retrieval and consider that endogenous IL-13 expression may be low; appropriate controls are recommended.

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