IL-1 alpha Rabbit pAb for WB, IHC-P, ELISA - P01583

Core Product Specifications and Parameters

Biological Background: IL-1 alpha Function and Localization

• Interleukin-1 alpha (IL-1α), also known as Hematopoietin-1, is encoded by the IL1A gene and belongs to the IL-1 family of cytokines.
• Constitutively expressed in nearly all resting non-hematopoietic cells, IL-1α functions as both an intracellular protein and a secreted cytokine that bridges innate and adaptive immunity. Related references: PMID:26439902
• Upon binding to IL1R1 and IL1RAP, it forms a high-affinity receptor complex, recruiting adaptors MYD88, IRAK1/4 and activating NF-κB, p38, p42/p44, and JNK signaling pathways. Related references: PMID:17507369, PMID:2950091, PMID:14687581
• Acts as an alarmin: passively released upon cell necrosis, alerting the host to tissue damage and triggering inflammation. Related references: PMID:15679580
• Intracellularly, IL-1α can directly sense DNA damage and serve as a signal for genotoxic stress without compromising cell membrane integrity. Related references: PMID:26439902
• Subcellularly localized to the nucleus, cytoplasm, and secreted into extracellular space, reflecting its dual intracellular/extracellular roles.
• Post-translational modifications include acetylation, glycosylation, lipidation (myristoylation), and phosphorylation, which may regulate its localization and activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 113-267) within the mature IL-1α protein, which may recognize both precursor and processed forms. For WB, consider a molecular weight around 31kDa (full-length precursor) and detect possible cleavage products.
• Given cross-reactivity with mouse and rat, this antibody can be utilized across species in comparative studies; however, we recommend validating in the relevant sample system.
• For IHC-P, optimize antigen retrieval and antibody dilution; since IL-1α is secreted and cytoplasmic/nuclear, assess staining patterns accordingly.
• For ELISA, consider pairing with a suitable detection antibody; this polyclonal pAb may serve as a capture or detection reagent after proper validation.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing partner for IVD raw materials, CamelBio streamlines the procurement of high-quality antibodies like this IL-1α polyclonal antibody, supporting inflammation and immunology research. We offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to bridge your workflow from concept to clinic. Contact us to discuss your specific assay development needs.