IL-1 alpha Rabbit mAb - P01582

Core Product Specifications and Parameters

Biological Background: IL-1 alpha Function and Localization

• Interleukin-1 alpha (IL-1α) is a cytokine constitutively present in nearly all resting non-hematopoietic cells and plays a critical role in inflammation, bridging innate and adaptive immunity. Related references: PMID:16256210
• Upon binding to its receptor IL1R1 and accessory protein IL1RAP, the high-affinity receptor complex recruits MYD88, IRAK1, and IRAK4, leading to activation of NF-κB and the p38, p42/p44, and JNK MAPK pathways. Related references: PMID:1386364
• IL-1α functions as an alarmin: cell death causes its passive release into the extracellular space, triggering inflammation and alerting the host to injury.
• The protein directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity.
• IL-1α localizes to the nucleus, cytoplasm, and is secreted; post-translational modifications include acetylation, phosphorylation, and glycosylation.
• The unprocessed precursor has a molecular weight of ~31 kDa (mature form ~17 kDa), consistent with its role as a pyrogen and mitogen.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 115–270 of mouse IL-1α) spans the mature cytokine region; the antibody may detect both the ~31 kDa pro-form and the ~17 kDa cleaved mature form in Western blot, depending on sample preparation.
• For Western blotting, consider using freshly prepared lysates with protease inhibitors and boiling under reducing conditions; validate detection of processed IL-1α in stimulated macrophage or epithelial cell samples.
• For ELISA, this monoclonal antibody can be explored as a capture or detection reagent in sandwich assays; pair with a second antibody targeting a distinct epitope and validate with recombinant mouse IL-1α standard.
• Cross-reactivity has been confirmed for mouse IL-1α; for other species or isoforms, assess sequence homology and perform experimental verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw materials, supporting diagnostic developers with a comprehensive portfolio from concept to clinical application. This IL-1 alpha rabbit monoclonal antibody exemplifies our offering in inflammation and cytokine research, backed by recombinant protein validation. We supply validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and can source rare-target raw materials to accelerate your assay development and manufacturing workflows.