IKKβ Rabbit Monoclonal Antibody for IF/ICC and ELISA - O14920

Core Product Specifications and Parameters

Biological Background: IKKβ Function and Localization

• IKKβ (Inhibitor of nuclear factor kappa-B kinase subunit beta), also known as IKK2 or NFKBIKB, is a serine/threonine protein kinase encoded by the IKBKB gene and is a core component of the canonical IKK complex that activates NF-κB signaling (PubMed:9346484). Related references: PMID:9346484

• It phosphorylates NF-κB inhibitors (IκB) on two critical serine residues, triggering their polyubiquitination and proteasomal degradation, thereby allowing NF-κB to translocate to the nucleus and activate gene transcription (PubMed:20434986, PubMed:20797629, PubMed:21138416). Related references: PMID:20434986, PMID:20797629, PMID:21138416

• Beyond IκB, IKKβ phosphorylates additional pathway components including NEMO/IKBKG, RELA, NFKB1, TBK1, and IKBKE, which can serve as a negative feedback mechanism to prevent overproduction of inflammatory mediators (PubMed:11297557, PubMed:14673179, PubMed:20410276). Related references: PMID:11297557, PMID:14673179, PMID:20410276

• The kinase also phosphorylates FOXO3, mediating TNF-dependent inactivation of this pro-apoptotic transcription factor (PubMed:15084260), and other substrates such as NAA10, NCOA3, BCL10, and IRS1 (PubMed:17213322, PubMed:19716809). Related references: PMID:15084260, PMID:17213322, PMID:19716809

• IKKβ is involved in IRF5 activation by phosphorylating its C-terminus to stimulate homodimerization and nuclear translocation (PubMed:25326418), and in response to LPS, it phosphorylates STAT1 at Thr-749, modulating inflammatory gene expression (PubMed:32209697, PubMed:38621137). Related references: PMID:25326418, PMID:32209697, PMID:38621137

• Subcellularly, IKKβ is found in the cytoplasm, nucleus, and membrane rafts. It is highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, and peripheral blood.

• Post-translational modifications include acetylation, hydroxylation, phosphorylation, S-nitrosylation, and ubiquitination, reflecting its tight regulation.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit monoclonal antibody covers amino acids 450-756, corresponding to the C-terminal region of human IKKβ. This design may provide specific recognition of full-length IKKβ in denatured or native conformations.
• For IF/ICC, consider using appropriate fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) protocols, and validate the antibody in IKKβ-expressing cell lines such as HeLa or HEK293.
• In ELISA applications, the antibody may be used for direct or sandwich detection; optimization of coating antigen and antibody dilution is recommended.
• Since the antibody is raised in rabbit, select compatible secondary antibodies for detection and consider potential cross-reactivity with endogenous immunoglobulins in tissue samples.
• Always include positive and negative controls (e.g., IKKβ knockout lysates or blocking peptides) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a premier IVD raw-material partner, CamelBio supports diagnostic manufacturers and researchers with a seamless supply chain from discovery to clinical application. This anti-IKKβ antibody exemplifies our commitment to providing high-quality tools for studying NF-κB signaling and inflammation. Our comprehensive portfolio includes validated antibody pairs for immunoassays, bulk recombinant proteins, and optimized monoclonal/polyclonal antibodies. We also excel in sourcing rare-target raw materials, ensuring you have reliable reagents for every stage of assay development and production.