IKKα Rabbit Monoclonal Antibody for WB, ELISA - O15111

Core Product Specifications and Parameters

Biological Background: IKKα (CHUK) Function and Localization

• IKKα (CHUK) is a serine/threonine-protein kinase that acts as the catalytic subunit of the IKK complex, playing a central role in the canonical NF-kappa-B signaling pathway by phosphorylating IκB inhibitors, triggering their ubiquitination and proteasomal degradation.
  Related references: PMID:18626576 PMID:9244310 PMID:9252186

• It also participates in the non-canonical NF-kappa-B pathway, where the MAP3K14-activated IKKα homodimer phosphorylates NFKB2/p100, inducing its processing to p52 and formation of RelB-p52 complexes that regulate B-cell survival and lymphoid organogenesis.
  Related references: PMID:20501937

• IKKα exerts negative feedback control on both canonical and non-canonical NF-kappa-B pathways: it promotes assembly of the A20 ubiquitin-editing complex to limit inflammatory gene activation, and also phosphorylates and destabilizes MAP3K14/NIK.
  Related references: PMID:21765415

• Within the nucleus, IKKα phosphorylates CREBBP, enhancing its transcriptional and histone acetyltransferase activities, and also phosphorylates histone H3 at Ser-10, facilitating subsequent acetylation at Lys-14 by CREBBP to modulate chromatin accessibility at NF-kappa-B-responsive promoters.
  Related references: PMID:17434128 PMID:12789342

• IKKα phosphorylates FOXO3, potentially regulating its pro-apoptotic activity.
  Related references: PMID:15084260

• In the context of mitophagy, IKKα phosphorylates AMBRA1, promoting its interaction with ATG8 family proteins and stimulating mitophagic activity.
  Related references: PMID:30217973

• The protein is widely expressed and localizes to both the cytoplasm and nucleus, consistent with its dual roles in signaling and transcriptional regulation.

• Key post-translational modifications include acetylation and ubiquitin conjugation, and IKKα contains an ATP-binding domain essential for its kinase function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 at the N-terminus of human IKKα; this region is likely conserved in mouse, supporting the reported cross-reactivity. Validate in the relevant sample system before large-scale experiments.
• For Western blot, an expected band at approximately 85kDa should be observed. Optimize antibody dilution and blocking conditions for your specific lysates.
• In ELISA applications, consider using the immunogen peptide as a positive coating antigen to confirm antibody reactivity, and test cross-reactivity with mouse IKKα if needed.
• Note that multiple isoforms or post-translationally modified forms may produce additional bands; consult current literature for the specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing for targets like IKKα in NF-kappa-B signaling. Partner with us for reliable supply and technical support throughout your diagnostic development journey.