IGFBP1 Rabbit pAb for WB, ELISA - P08833

Core Product Specifications and Parameters

Biological Background: IGFBP1 Function and Localization

• Insulin-like growth factor-binding protein 1 (IGFBP1), also known as placental protein 12 (PP12), is a secreted multifunctional protein encoded by the IGFBP1 gene (Gene ID: 3484).
• IGFBP1 critically regulates the bioavailability of insulin-like growth factors IGF1 and IGF2, thereby modulating IGF-mediated cellular processes such as migration, proliferation, differentiation, and apoptosis in a cell-type specific manner.

Related references: PMID:11397844 PMID:15972819

• The protein promotes cell migration through its RGD motif, which interacts with integrin ITGA5:ITGB1, leading to activation of focal adhesion kinase/PTK2 and the MAPK pathway.

Related references: PMID:7504269 PMID:11397844

• In cardiomyocytes, IGFBP1 suppresses HIF-1α/HIF1A ubiquitination and degradation, thereby regulating apoptosis (By similarity).
• Post-translational modifications include phosphorylation and the presence of disulfide bonds, which contribute to its structural stability and functional regulation.
• Subcellularly, IGFBP1 is secreted and functions primarily in the extracellular space, consistent with its role as a carrier protein for IGFs.

Experimental Guidance and Technical Tips

• The immunogen spans the central region (aa 81-259) of human IGFBP1, encompassing both the IGF-binding and integrin-recognition domains; therefore, the antibody may detect functionally intact forms in WB and ELISA.
• For Western blot, consider running samples under both reducing and non-reducing conditions to account for disulfide-bonded structures; a band near 28 kDa is anticipated for monomeric IGFBP1.
• In ELISA, validate the antibody as part of a matched pair using recombinant or native IGFBP1 in your specific sample matrix to ensure optimal assay performance.
• Given the cross-reactivity with mouse and rat, this antibody can be employed in comparative studies across species, but always confirm specificity in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined procurement experience for IVD raw materials, technical services, and consulting from concept to clinic. This high-quality anti-IGFBP1 rabbit polyclonal antibody supports growth factor research and metabolic studies. Our portfolio also includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized raw-material sourcing for rare targets, empowering your IVD development pipeline.