IGF2BP3 Rabbit Polyclonal Antibody (WB, IP, IF, IHC-P, ELISA) - O00425

Core Product Specifications and Parameters

Biological Background: IGF2BP3 Function and Localization

• Also known as IMP3 and VICKZ3, belongs to the VICKZ family of mRNA-binding proteins.
• Functions as an RNA-binding protein that recruits target transcripts to cytoplasmic mRNA-protein complexes (mRNPs) for transport, transient storage, and translational regulation.
• Preferentially binds to N6-methyladenosine (m6A)-modified mRNAs, enhancing their stability; also stabilizes MYC mRNA by binding to the coding region instability determinant (CRD) in an m6A-dependent manner. Related references: PMID:29476152
• Binds to the 3'-UTR of CD44 mRNA, stabilizing it and promoting cell adhesion and invadopodia formation in cancer cells.
• Binds to beta-actin/ACTB and the 5'-UTR of insulin-like growth factor 2 (IGF2) mRNAs, regulating their expression.
• Localizes to the nucleus, cytoplasm, P-bodies, and stress granules, reflecting roles in mRNA processing, storage, and stress response.
• Expressed in fetal tissues (including liver, lung, kidney, thymus, placenta) and overexpressed in cervical, testicular, pancreatic, and renal cell carcinomas.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitination (based on UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 300-579 of human IGF2BP3 (NP_006538.2), a region conserved in mouse, consistent with the reported cross-reactivity.
• For Western blotting, a band of approximately 64 kDa is expected; use appropriate positive and negative controls to validate specificity.
• For immunoprecipitation (IP), standard protocols may be used; verify capture efficiency by co-detection of known interacting mRNAs or proteins.
• For immunofluorescence/immunocytochemistry (IF/ICC) and immunohistochemistry (IHC-P), optimize fixation and permeabilization; nuclear and cytoplasmic staining may be observed.
• Consider using the antibody in ELISA with the immunogen peptide as a positive control; titration experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-IGF2BP3 polyclonal antibody is part of a broad portfolio for cancer and mRNA biology studies. Beyond individual antibodies, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and assists in sourcing rare target raw materials—ensuring a reliable supply chain for your assay development needs.