IGF2BP1/IMP1 Rabbit mAb for WB, IHC-P, IF/ICC, IP, ELISA - Q9NZI8

Core Product Specifications and Parameters

Biological Background: IGF2BP1/IMP1 Function and Localization

• Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1/IMP1) is an RNA-binding protein belonging to the VICKZ family, encoded by the IGF2BP1 gene. Also known as CRD-BP, ZBP1, or VICKZ1, it features multiple RNA-binding domains that recognize specific mRNA elements.
• Core function: Recruits target transcripts into cytoplasmic mRNP complexes, enabling mRNA transport, temporary storage, and translational repression. Preferentially binds N6-methyladenosine (m6A)-modified mRNAs, enhancing their stability. Related references: PMID:29476152, PMID:32245947
• Plays a key role in spatial regulation of ACTB/β-actin mRNA: binds the ‘zipcode’ element, transports the mRNP along the cytoskeleton, and releases the transcript upon phosphorylation, allowing local actin synthesis essential for cell polarity and migration.
• During neuronal development, IGF2BP1 regulates neurite outgrowth, growth cone guidance, and neuronal migration by spatiotemporally controlling protein synthesis of cargo mRNAs like ACTB.
• Binds the coding region instability determinant (CRD) of MYC mRNA, shielding it from endonucleolytic cleavage. This interaction is potentiated by m6A modification in the CRD. Related references: PMID:29476152
• Also stabilizes and regulates translation of other cancer-associated transcripts (e.g., CD44, PTEN, IGF2, BTRC), modulates stress granule recruitment during oxidative stress, and interacts with viral RNAs (HCV IRES, HIV-1 GAG) to either promote or inhibit viral replication.
• Subcellular localization includes the nucleus, cytoplasm, perinuclear region, P-bodies, stress granules, and cell projections such as lamellipodia, dendrites, growth cones, and axons, reflecting its dynamic roles in mRNA transport and local translation.
• Mainly expressed during embryogenesis (fetal liver, lung, kidney, thymus) and in reproductive tissues (ovary follicles, testis, placenta). Overexpressed in multiple cancers, including testis, lung, kidney, prostate, and trachea, making it a potential biomarker and therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an N-terminal region (amino acids 101–200) of human IGF2BP1; consider sequence alignment to verify reactivity with isoforms or species homologs.
• For Western blotting, predicted full-length molecular weight is ~63 kDa; the datasheet lists 49 kDa/63 kDa. Use positive controls from cell lines known to express IGF2BP1 (e.g., embryonic or cancer lines) and validate band identity.
• In IHC-P and IF/ICC, IGF2BP1 localizes to both nucleus and cytoplasm, with potential enrichment in cell protrusions. Optimize permeabilization and include isotype controls.
• For immunoprecipitation, standard protocols may require optimization of salt and detergent concentrations to preserve native complexes.
• For ELISA, the antibody can be used as a capture or detection reagent; consider pairing with a second antibody targeting a distinct epitope for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutes with streamlined access to validated IVD raw materials. From recombinant antibodies and optimized monoclonal pairings to bulk ancillary reagents, we cover every stage from concept to clinic. For projects targeting RNA-binding proteins or cancer pathways, explore our IGF2BP1/IMP1 antibody and related immunoreagents to accelerate your assay development.