IFN gamma Rabbit PolymAb® for WB and ELISA - P01579 / P01581

Core Product Specifications and Parameters

Biological Background: IFN gamma Function and Localization

• Interferon gamma (IFN-γ), encoded by the IFNG gene, is a type II interferon and a key secreted cytokine belonging to the interferon family. It is also known as immune interferon (If2f; IFNG2) and is primarily released from activated T lymphocytes.

• It plays a critical role in antimicrobial, antiviral, and antitumor immune responses by activating effector immune cells and enhancing antigen presentation. Related references: PMID:16914093, PMID:8666937

• IFN-γ signals through the JAK-STAT pathway upon binding to its receptor IFNGR1, leading to activation of STAT1 and transcription of IFN-γ-regulated genes, many of which are transcription factors like IRF1 that further amplify the immune response. Related references: PMID:16914093, PMID:8349687

• It enhances antigen presentation by inducing the replacement of constitutive proteasome subunits with immunoproteasome subunits, thereby increasing the quantity, quality, and repertoire of peptides for MHC class I loading. Related references: PMID:8666937, PMID:8163024

• IFN-γ also upregulates MHC class II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Related references: PMID:7729559

• It increases the efficiency of peptide generation by inducing the activator PA28, which associates with the proteasome and alters its proteolytic cleavage preference. Related references: PMID:11112687

• Post-translationally, IFN-γ is a glycoprotein with a signal peptide, and it undergoes cleavage on pairs of basic residues and contains a pyrrolidone carboxylic acid modification.

• Under homeostatic conditions, IFN-γ participates in the regulation of hematopoietic stem cells, influencing their development, quiescence, and differentiation.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted molecular weight of IFN-γ is approximately 18 kDa; consider using reducing conditions and validate in the relevant sample system (e.g., stimulated T-cell lysates or recombinant protein controls).
• In ELISA applications, this antibody may be used as a detection or capture antibody; pairing with a validated matched antibody pair is recommended and requires individual optimization.
• Since the immunogen is a recombinant protein covering human and rat IFN-γ, cross-reactivity with other species should be verified experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, recombinant proteins, and bulk ancillary reagents to accelerate diagnostic development. For interferon gamma research, explore our portfolio of monoclonal antibodies and complementary reagents optimized for immunological assays. Our team can also assist with rare target raw-material sourcing to meet your specific project needs.