ICAM-1/CD54 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P13597

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• Encoded by the Icam1 gene, ICAM-1 (CD54) is a cell surface glycoprotein belonging to the immunoglobulin superfamily, with a molecular weight of approximately 59 kDa (58.8 kDa calculated).
• Functions as a critical adhesion molecule by serving as a receptor for integrins ITGAL:ITGB2 (LFA-1) and ITGAM:ITGB2 (MAC-1), mediating leukocyte cell-cell adhesion and neutrophil-endothelial interactions.
• Provides a calibrated system to adjust T-cell killing to antigen stimulation strength via LFA-1 binding.
• During leukocyte trans-endothelial migration, ICAM-1 engagement promotes endothelial apical cup assembly through ARHGEF26/SGEF and RHOG activation.
• In epithelial cells, interaction with MUC1 promotes cell aggregation.
• Localizes to the cell membrane as a single-pass type I transmembrane protein.
• Basal expression is low on lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells and on fibroblasts and epithelial cells by inflammatory stimuli.
• Contains multiple immunoglobulin-like domains, disulfide bonds, and N-linked glycosylation sites; undergoes alternative splicing and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• For Western blot, a band near 59 kDa is expected; verify specificity using appropriate positive and negative controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), optimize antigen retrieval and permeabilization conditions for your sample type.
• Cross-reactivity with rat allows for comparative studies; confirm reactivity in rat tissues with appropriate controls.
• ELISA application may be suitable for detecting soluble or recombinant ICAM-1; consider using a validated coating buffer.
• As a rabbit monoclonal antibody, it offers batch-to-batch consistency; store as recommended to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this ICAM-1/CD54 Rabbit Monoclonal Antibody as part of its comprehensive IVD raw-material portfolio, supporting researchers studying cell adhesion and immune cell trafficking. From validated antibody pairs to optimized monoclonal/polyclonal antibodies and bulk ancillary reagents, CamelBio streamlines procurement for diagnostic manufacturers and laboratories. Explore our one-stop sourcing services for rare targets and customized raw-material solutions.