Hydroxyl-Histone H2A-Y39 (P04908/P0C0S8) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H2A Function and Localization

Histone H2A is a core component of the nucleosome, responsible for wrapping and compacting DNA into chromatin, thereby regulating DNA accessibility for transcription, repair, replication, and maintaining chromosomal stability. The protein exists as several variants, encoded by multiple genes including H2AC4, H2AC8 (P04908), and H2AC11, H2AC13, H2AC15, H2AC16, H2AC17 (P0C0S8). It is localized exclusively in the nucleus and on chromosomes.

Post-translational modifications (PTMs) of histones, collectively known as the "histone code," play crucial roles in epigenetic regulation. Histone H2A is subject to a wide array of PTMs, including acetylation, methylation, phosphorylation, ubiquitylation, and hydroxylation, among others. Modification-specific antibodies, such as this rabbit monoclonal antibody, enable detection of specific PTM states like hydroxylation at tyrosine 39 (Y39), providing insights into chromatin dynamics.

Related references:

• UniProt P04908
• UniProt P0C0S8

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic hydroxylated peptide corresponding to residues around Y39 of human histone H2A (P04908). Confirm that the specific modification is present in your experimental system, as PTM levels can vary with cell type and treatment conditions.
• For Western blot (WB), a band at approximately 14 kDa is expected, consistent with the predicted molecular weight of histone H2A. Consider using nuclear extracts or acid-extracted histones for enriched detection.
• For immunohistochemistry (IHC-P), include appropriate controls such as peptide blocking to verify staining specificity. Optimize antigen retrieval and antibody dilution for your tissue samples.
• In ELISA applications, pre-coat with the modified peptide or recombinant histone to establish a standard curve; include non-modified peptide as a negative control.
• Cross-reactivity is anticipated with human, mouse, rat, and other species based on sequence homology; however, validate for each new species to ensure reliable performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This hydroxyl-Histone H2A-Y39 rabbit monoclonal antibody is part of our expanding catalog of modification-specific antibodies for epigenetics research. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, ensuring you have the critical raw materials needed for assay development and scale-up.