Human IgM Rabbit pAb for WB, ELISA - P01871

Core Product Specifications and Parameters

Biological Background: Human IgM Function and Localization

• IGHM encodes the constant region of the immunoglobulin mu heavy chain, forming IgM antibodies. Membrane-bound IgM serves as the B cell receptor (BCR) that triggers clonal expansion and differentiation upon antigen binding, while secreted IgM mediates pathogen elimination (PubMed:20176268, PubMed:22158414). Related references: PMID:20176268, PMID:22158414

• Secreted IgM (sIgM) multimerizes into pentamers or hexamers, providing high-avidity antigen recognition and avidity (PubMed:32029689, PubMed:37095205). Related references: PMID:32029689, PMID:37095205

• sIgM effector functions include binding to the high-affinity Fc receptor FCMR on lymphoid cells, promoting neutralizing IgG responses while maintaining self-tolerance. It also recruits C1q to activate the classical complement pathway and facilitates phagocytosis of apoptotic cells (PubMed:12847249, PubMed:19006321, PubMed:28230186). Related references: PMID:12847249, PMID:19006321, PMID:28230186

• IgM plays a role in mucosal immunity: it undergoes transcytosis across mucosal epithelium via PIGR and is secreted into mucosal linings. IgM-antigen complexes undergo FCMR-mediated retrotranscytosis across M cells to underlying lymphoid tissues (PubMed:32029689). Related references: PMID:32029689

• Membrane-bound IgM is a component of the B cell receptor complex, providing constitutive tonic signaling essential for B cell survival and mediating pre-BCR signaling for allelic exclusion and gene rearrangement.

• Subcellularly, IGHM products are either secreted or anchored as single-pass type I membrane proteins on the cell surface.

• As a glycoprotein with immunoglobulin domains, IgM contains intrachain and interchain disulfide bonds that stabilize its structure, and it undergoes post-translational glycosylation.

Experimental Guidance and Technical Tips

• The immunogen covers the constant region (aa 213-453) of human IGHM, ensuring recognition of both soluble and membrane-bound IgM isoforms.
• For Western blotting, the IgM heavy chain migrates at approximately 70 kDa under reducing conditions due to glycosylation and the variable region; multimers observed under non-reducing conditions may exceed 900 kDa. Optimize sample preparation and gel percentage accordingly.
• In ELISA, this antibody can be employed as a capture or detection reagent for human IgM. Pairing with a monoclonal anti-human IgM detection antibody may enhance assay specificity.
• As a polyclonal antibody, lot-to-lot consistency should be verified for quantitative applications; include a control sample in each run.
• Store according to the manufacturer's recommendations (typically −20 °C) and minimize freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop supply of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This human IgM polyclonal antibody is part of our extensive immunology portfolio, supporting researchers in humoral immunity and B cell studies. Our expert sourcing team can assist with rare target raw materials and custom development services to accelerate your diagnostic or research workflows.