Human IgG (Fc) Rabbit Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - P01857 / P01859 / P01860 / P01861

Core Product Specifications and Parameters

Biological Background: IgG Fc Constant Region Function and Localization

• Immunoglobulin heavy constant gamma 1 (IgG1) is one of four human IgG subclasses (IgG1, IgG2, IgG3, IgG4), each encoded by distinct IGHG genes (IGHG1, IGHG2, IGHG3, IGHG4).
• The constant region of IgG1 mediates key humoral effector functions, including antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and phagocytosis, via interaction with Fc receptors on innate immune cells. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• Membrane-bound immunoglobulins serve as B-cell receptors (BCR); upon antigen binding, they trigger clonal expansion and differentiation of B lymphocytes into antibody-secreting plasma cells. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• Secreted antibodies block pathogen interactions and recruit immune effector mechanisms through their constant region, while the variable domains confer antigen specificity. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• Class-switch recombination diversifies the constant region, allowing B cells to switch from IgM/IgD to IgG, IgA, or IgE, thereby tailoring effector functions to the immune context. Related references: PMID:17576170, PMID:20176268, PMID:22158414
• IgG1 is present both as a secreted protein and as a membrane-bound receptor; alternative splicing generates the two forms, and the membrane form includes a transmembrane helix.
• Post‑translational modifications include glycosylation and disulfide bond formation, which are critical for proper folding and Fc receptor binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the Fc region of human IgG1 (amino acids 100–330, P01857); due to high sequence homology, the antibody may cross‑react with IgG2, IgG3, and IgG4. Validate subclass specificity if required.
• In Western blot (WB), under reducing conditions a band of approximately 52 kDa is detected for the heavy chain; under non‑reducing conditions, the whole IgG (~150 kDa) may be observed.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF‑P), consider antigen retrieval methods and appropriate fixation to preserve the Fc epitope.
• In ELISA, this antibody can be used as a capture or detection reagent for human IgG quantification; pair with matched secondary antibodies or antigen standards.
• As a rabbit monoclonal antibody, it typically offers high reproducibility and affinity, but performance should be verified in your specific sample matrix and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing for IVD raw materials, including this Human IgG (Fc) Rabbit mAb, to support your diagnostic assay development for IgG detection. We supply validated antibody pairs, monoclonal and polyclonal reagents, and bulk ancillary materials, facilitating efficient procurement from concept to clinic with reliable, research‑grade quality.