Human IgA1 Rabbit Monoclonal Antibody for WB and ELISA - P01876

Core Product Specifications and Parameters

Biological Background: IgA1 Function and Localization

• IGHA1 encodes the constant region of the immunoglobulin alpha-1 heavy chain, a core component of IgA1 antibodies essential for humoral immune effector functions.
  Related references: PMID:20176268, PMID:22158414
• IgA1 exists in two forms: membrane-bound on B lymphocytes as part of the B cell receptor, and secreted into mucosa and serum to neutralize pathogens.
• The variable domain of IgA1 undergoes V(D)J recombination and somatic hypermutation, enabling high-affinity antigen recognition and binding.
  Related references: PMID:17576170, PMID:20176268
• As the predominant immunoglobulin in mucosal secretions, IgA1 plays a critical role in first-line defense at respiratory, gastrointestinal, and urogenital surfaces.
  Related references: PMID:2241915
• Subcellularly, IgA1 is either secreted extracellularly or anchored on the B cell plasma membrane via a transmembrane helix, depending on alternative splicing.
• The protein features conserved immunoglobulin domains, numerous disulfide bonds, and glycosylation sites that influence its structural stability and function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 99–353 of human IgA1, covering most of the constant region; this design should enable recognition of both native and recombinant full-length IgA1.
• In Western blot, bands at ~37 kDa and ~42 kDa are likely observed due to differential glycosylation; inclusion of deglycosylation controls or use of glycosidase-treated lysates may help confirm band identity.
• For ELISA, consider using purified human IgA1 or relevant biological fluids (e.g., saliva, serum) as the antigen source; optimize coating buffer and blocking conditions for best signal-to-noise ratio.
• Validate the antibody in the sample system of interest (e.g., mucosal tissue lysates, B cell lines) and assess cross-reactivity with other immunoglobulin subclasses if working with complex mixtures.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including this high-quality anti-human IgA1 monoclonal antibody. Our platform simplifies procurement of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials for mucosal immunity and immunoglobulin research. With comprehensive technical support, CamelBio bridges the gap from concept to clinical application.