HSPB11 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y547

Core Product Specifications and Parameters

Biological Background: HSPB11 Function and Localization

• Intraflagellar transport protein 25 homolog (IFT25), also known as heat shock protein beta-11 (HSPB11) and placental protein 25 (PP25), is encoded by the IFT25/HSPB11 gene.
• Component of the IFT complex B required for sonic hedgehog (SHH) signaling; mediates transport of SHH components by facilitating export of SMO and PTCH1 receptors from the cilium and accumulation of GLI2 at the ciliary tip.
• Essential for male fertility, spermiogenesis, and sperm flagella formation.
• Plays a role in early kidney development, possibly involved in the regulation of ureteric bud initiation.
• Localizes to the cilium, a cell projection, consistent with its role in intraflagellar transport.
• Detected in placenta, with functional roles primarily in ciliated tissues such as kidney and testis.
• Associated with calcium and metal ion binding, stress response, differentiation, and protein transport.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-144) of human HSPB11, potentially recognizing conserved epitopes; cross-reactivity with mouse and rat is confirmed—validate in your specific sample system.
• For Western blotting (WB), the predicted molecular weight is ~16 kDa; use positive controls from testis, kidney, or placental lysates and confirm band specificity.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), target cilium localization; use ciliated tissues (e.g., kidney, testis) and optimize fixation/antigen retrieval protocols.
• For ELISA, the antibody can be used as a capture or detection reagent; pair with appropriate standards for quantitative assays.
• Always include appropriate negative and positive controls, and validate the antibody in the intended experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibodies, recombinant proteins, and ancillary reagents for research and diagnostic development. The HSPB11 polyclonal antibody supports studies in ciliary biology, SHH signaling, and male fertility, reflecting our commitment to supplying high-quality tools for rare and emerging targets. Our extensive portfolio and technical support help streamline your workflow from concept to clinic.