HSPA14 Rabbit mAb for WB, IHC-P, IF/ICC, ELISA - Q0VDF9

Core Product Specifications and Parameters

Biological Background: HSPA14 Function and Localization

• HSPA14 is a 55 kDa cytosolic molecular chaperone (heat shock 70 kDa protein 14) that functions as a component of the ribosome-associated complex (RAC).
• It binds to nascent polypeptide chains emerging from the ribosome, assisting in their folding and preventing aggregation.
• Together with its co-chaperone DNAJC2, which stimulates its ATPase activity, HSPA14 maintains newly synthesized proteins in a folding-competent state.
• The protein contains a nucleotide-binding domain that hydrolyzes ATP to drive conformational changes required for substrate binding and release.
• Localized in the cytoplasm and cytosol, HSPA14 is part of the cellular protein quality-control machinery.
• Alternative names include HSP70L1, HSP70-4, and HSP60, reflecting its membership in the HSP70 chaperone family.

Experimental Guidance and Technical Tips

• For Western blot, the predicted band is ~55 kDa; consider using a positive control such as HeLa or HEK293 lysate.
• In IHC-P and IF/ICC, validate in human tissue sections or cell lines; note that HSPA14 is cytosolic, so signal should be diffuse cytoplasmic.
• ELISA may be used for detection of recombinant or endogenous HSPA14 in solution.
• Since the immunogen is a peptide from the N-terminal region (aa 1‑100), the antibody likely recognizes both full-length and N-terminal fragments; confirm by peptide competition or knockdown.
• Cross-reactivity with mouse suggests potential utility in rodent models; validate in the relevant mouse tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable supply of high-quality IVD antibodies and ancillary reagents. This HSPA14 rabbit monoclonal antibody exemplifies our expanding catalog of chaperone-targeting reagents for protein folding and stress response studies. From validated antibody pairs to bulk raw materials, we streamline your sourcing—from concept to clinic.