HSP27/HSPB1 Rabbit pAb - P04792

Core Product Specifications and Parameters

Biological Background: HSP27/HSPB1 Function and Localization

• HSP27/HSPB1 (Heat shock protein beta-1), also known as HSP27, HSP28, Hsp25, SRP27, is a member of the small heat shock protein (sHSP) family. It acts as a molecular chaperone, maintaining denatured proteins in a folding-competent state. Related references: PMID:10383393 PMID:20178975
• It plays a role in stress resistance and actin organization, contributing to cellular protection under stress. Related references: PMID:19166925
• Through its chaperone activity, HSP27 regulates the phosphorylation and axonal transport of neurofilament proteins, which is important for neuronal function. Related references: PMID:23728742
• The protein localizes to the cytoplasm, nucleus, and cytoskeleton (spindle), reflecting its dynamic role in various cellular compartments.
• It is ubiquitously expressed, with highest levels in heart and striated/smooth muscle tissues. It is detected in skeletal muscle, aorta, intestine, stomach, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum, and cerebrospinal fluid.
• HSP27 undergoes various post-translational modifications, including phosphorylation, acetylation, and methylation, which regulate its function.
• Mutations in HSPB1 are associated with Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy, linking it to neurodegenerative disorders.
• Additional keywords: 3D-structure, Chaperone, Host-virus interaction, Stress response, Phosphoprotein, Reference proteome.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated against an immunogen comprising amino acids 1-100 of human HSP27/HSPB1, a region that may be conserved across species. Validate species cross-reactivity in your own experimental system before large-scale studies.
• For Western blot (WB), HSP27/HSPB1 has a predicted molecular weight of 23 kDa. Use appropriate positive controls (e.g., HeLa, HEK293 cells) and optimize loading amounts.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and antibody dilution should be optimized based on tissue type and fixation method. Consider testing both cytoplasmic and nuclear staining patterns.
• For ELISA, pair the antibody with a suitable detection antibody and validate sensitivity and specificity in your assay setup.

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CamelBio is a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies like this HSP27/HSPB1 Rabbit pAb, and bulk ancillary reagents. We specialize in supplying rare targets and supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. Let us bridge your stress response and chaperone research with reliable raw materials.