Hsc70/HSPA8 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P11142

Core Product Specifications and Parameters

Biological Background: Hsc70/HSPA8 Function and Localization

• Hsc70/HSPA8 is a 71 kDa heat shock cognate protein, also known as HSP73, HSPA10, and LAP-1, encoded by the HSPA8 gene.
• It functions as a molecular chaperone, essential for protein folding, proteome protection, and chaperone-mediated autophagy (CMA). Related references: PMID:21148293 PMID:21150129 PMID:23018488
• Its chaperone activity is driven by ATP hydrolysis, cycling between low-affinity ATP-bound and high-affinity ADP-bound states, regulated by co-chaperones. Related references: PMID:12526792 PMID:21148293 PMID:21150129 PMID:24121476 PMID:24318877 PMID:26865365
• In CMA, Hsc70 recognizes cytosolic proteins with a KFERQ motif, targeting them to lysosomal LAMP2 for degradation. Related references: PMID:11559757 PMID:2799391 PMID:36586411
• Hsc70 binds bacterial lipopolysaccharide (LPS) and mediates TNF secretion by monocytes, linking it to innate immunity. Related references: PMID:10722728 PMID:11276205
• Subcellular localization includes cytoplasm, nucleus, melanosomes, lysosomal membrane, and cell membrane.
• It is subject to multiple post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination.
• Ubiquitously expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 510–615, a region likely conserved across species; verify sequence homology if testing non-listed organisms.
• For Western blotting, expect a band near 71 kDa; modified forms may appear at slightly different molecular weights.
• In IHC-P and IF/ICC, optimization of antigen retrieval and blocking conditions is recommended for each tissue or cell type.
• For ELISA, consider testing serial dilutions of the antibody to establish optimal working concentrations.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, such as this anti-Hsc70/HSPA8 polyclonal antibody. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance with rare-target sourcing, our one-stop supply chain spans from concept to clinic. We support your studies in protein homeostasis, autophagy, and stress response with tailored procurement solutions.