HS2ST1 Rabbit pAb - Q7LGA3

Core Product Specifications and Parameters

Biological Background: HS2ST1 Function and Localization

• HS2ST1 encodes heparan sulfate 2-O-sulfotransferase 1, a Golgi-resident enzyme responsible for the 2-O-sulfation of iduronic acid and glucuronic acid residues within heparan sulfate (HS) chains.
• The enzyme catalyzes the transfer of a sulfo group from 3'-phospho-5'-adenylyl sulfate (PAPS) to the 2-OH position of iduronic acid (IdoA) or glucuronic acid (GlcA) in nascent HS, playing a critical role in HS structural maturation and biological activity.
• This 2-O-sulfation modification is essential for proper kidney development; loss of HS2ST1 function disrupts ureteric bud-metanephric mesenchyme signaling, leading to impaired metanephric formation.
• HS2ST1 is a type II transmembrane protein anchored to the Golgi apparatus membrane, featuring a short cytoplasmic N-terminus, a signal-anchor transmembrane helix, and a large luminal catalytic domain with multiple disulfide bonds and glycosylation sites.
• The protein is predicted to form a coiled-coil region and undergoes alternative splicing, giving rise to multiple isoforms.
• Clinically, mutations in HS2ST1 have been associated with autosomal recessive intellectual disability, underscoring the importance of HS sulfation patterns in neurodevelopment.
• Uniprot lists the molecular weight at approximately 41881 Da, consistent with the observed band at 42 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–229 of human HS2ST1, a region likely to recognize multiple spliced isoforms; consider this when interpreting banding patterns in western blot.
• For WB, a band around 42 kDa is expected in human, mouse, and rat samples; tissue extracts from kidney or neural sources may yield stronger signals due to higher endogenous expression.
• ELISA applications can be used to quantitate HS2ST1 levels or to screen hybridoma supernatants; validate in the relevant sample system for your assay.
• The antibody’s cross-reactivity with mouse and rat enables translational models; however, verify performance under your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD manufacturers and research laboratories, offering a comprehensive portfolio of raw materials from early concept to clinical application. This HS2ST1 rabbit pAb supports your work in heparan sulfate biology, glycobiology, and related disease research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials—ensuring you have everything needed for robust assay development.