HRSP12 Rabbit Polyclonal Antibody for WB, ELISA - P52758

Core Product Specifications and Parameters

Biological Background: HRSP12 Function and Localization

• Encoded by the RIDA (HRSP12) gene, the protein is known as 2-iminobutanoate/2-iminopropanoate deaminase, also referred to as 14.5 kDa translational inhibitor protein (hp14.5), heat-responsive protein 12, reactive intermediate imine deaminase A homolog, translation inhibitor L-PSP ribonuclease, and UK114 antigen homolog.
• Primary function: Catalyzes the hydrolytic deamination of enamine/imine intermediates produced during normal metabolism, potentially reducing cellular toxicity from reactive metabolites.
• RNA-related function: Promotes endoribonucleolytic cleavage of certain transcripts by bridging YTHDF2 and the ribonuclease P/MRP complex. This activity targets N6-methyladenosine (m6A)-containing mRNAs with a 5′-GGUUC-3′ motif, leading to their degradation. Related references: PMID:30930054 PMID:8973653
• Subcellular localization: Found in the cytoplasm, nucleus, peroxisome, and mitochondrion.
• Tissue specificity: Highly expressed in liver and kidney, with lower levels detected in lung and brain.
• Post-translational modifications and structural features: Acetylated, phosphorylated, and directly sequenced at the protein level; molecular weight is 14,494 Da according to UniProt.
• Keyword annotations: 3D-structure, Acetylation, Cytoplasm, Direct protein sequencing, Hydrolase, Lipid metabolism, Mitochondrion, Nucleus, Peroxisome, Phosphoprotein, Proteomics identification, Reference proteome, RNA-binding.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human HRSP12 protein (amino acids 1–137), which may allow detection of the endogenous target across human, mouse, and rat samples in WB and ELISA.
• In Western blotting, expect a band near 14 kDa; consider including positive controls (e.g., liver or kidney lysates) and verifying specificity with knockdown or knockout models if available.
• For ELISA applications, test optimal coating concentrations and antibody dilutions experimentally; cross-reactivity with mouse and rat orthologs may be useful for comparative studies.
• Since HRSP12 localizes to multiple compartments, fractionation experiments could help confirm its subcellular distribution in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with seamless access to validated IVD raw materials, including this HRSP12 rabbit polyclonal antibody for metabolism and RNA biology applications. Our portfolio spans optimized monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents, backed by technical support from concept to clinic. Whether you need bulk sourcing of this antibody, customized formats, or rare target raw materials, CamelBio streamlines procurement so you can focus on your assay development.