HOXB5 Rabbit pAb for WB, IF/ICC, ELISA - P09067

Core Product Specifications and Parameters

Biological Background: HOXB5 Function and Localization

• HOXB5, also known as Homeobox protein HHO.C10, Hox-2A, and Hu-1, is a transcription factor belonging to the HOX gene family.
• It acts as a sequence-specific transcription factor that provides cells with specific positional identities along the anterior-posterior axis during embryonic development.
• The protein contains a conserved homeobox domain, facilitating DNA binding and transcriptional regulation.
• HOXB5 is localized in the nucleus, consistent with its function in gene expression control.
• It is predominantly expressed in the spinal cord, as indicated by tissue specificity data.
• Annotated with keywords including developmental protein, DNA-binding, homeobox, nucleus, and transcription regulation, underscoring its role in development.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 170-269 of human HOXB5 (NP_002138.1). Consider verifying sequence homology between your species of interest and the immunogen for optimal recognition.
• For Western blotting, a band around 29 kDa is anticipated in lysates from human, mouse, and rat samples. Include appropriate positive controls (e.g., spinal cord tissue) and molecular weight markers.
• In immunofluorescence (IF/ICC), nuclear localization should be observed. Use appropriate fixation and permeabilization protocols, and include nuclear counterstains to confirm subcellular distribution.
• For ELISA applications, this polyclonal antibody can serve as either capture or detection reagent. Optimize coating concentrations and detection systems for your specific assay format.
• Cross-reactivity has been reported for human, mouse, and rat. If using other species, validate species cross-reactivity experimentally before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you need rare-target raw materials for HOXB5-related developmental biology or technical consulting to accelerate your diagnostic development, CamelBio supports every stage from concept to clinic.