HOPX Rabbit PolymAb® for WB, IP, IF-P, IHC-P, ELISA - Q9BPY8

Core Product Specifications and Parameters

Biological Background: HOPX (Homeodomain-only protein) Function and Localization

• HOPX is an atypical homeodomain protein that lacks DNA-binding activity and functions as a transcriptional repressor.
• It modulates cardiac growth and development by interacting with serum response factor (SRF), inhibiting SRF-dependent transcription either by blocking DNA binding or recruiting histone deacetylases (HDACs).
• Overexpression of HOPX can cause cardiac hypertrophy.
• HOPX may act as a tumor suppressor; it is down-regulated in several cancers including lung cancer, choriocarcinoma, head and neck squamous cell carcinoma, and oral squamous cell carcinoma.
• It functions as a co-chaperone for HSPA1A and HSPA1B, assisting in chaperone-mediated protein refolding. Related references: PMID:27708256
• HOPX localizes to both the nucleus and cytoplasm.
• Alternative splicing produces multiple isoforms, and it contains a characteristic homeobox domain.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–73) of human HOPX. This region is conserved across species, consistent with the observed cross-reactivity with mouse and rat.
• For western blotting, expect a band at approximately 8 kDa. Use a low-molecular-weight marker and optimize transfer conditions for small proteins.
• In immunoprecipitation, pre-clear lysates and include appropriate negative controls to minimize background.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), validate antigen retrieval and permeabilization protocols in your specific tissue or cell system.
• The antibody performs well in ELISA applications; consider pairing with a suitable detection reagent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-HOPX antibody is part of our extensive portfolio supporting cardiac biology and oncology research. We supply validated monoclonal/polyclonal antibodies, matched antibody pairs, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to accelerate your assay development and biomarker programs.