HOMER1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q86YM7

Core Product Specifications and Parameters

Biological Background: HOMER1 Function and Localization

• HOMER1 is a postsynaptic density scaffolding protein that belongs to the Homer family, encoded by the HOMER1 gene (Gene ID: 9456) and also known as SYN47.
• It binds and cross-links cytoplasmic regions of metabotropic glutamate receptors (GRM1, GRM5), inositol 1,4,5-trisphosphate receptor (ITPR1), ryanodine receptors (RYR1, RYR2), and scaffold proteins (SHANK1, SHANK3), coupling surface receptors to intracellular calcium release.
• Isoform 1 (canonical) regulates trafficking and surface expression of GRM5, while isoform 3 acts as a dominant negative, competing with isoform 1 to modulate synaptic metabotropic glutamate signaling, thereby influencing long-term neuronal plasticity and development.
• HOMER1 forms high-order complexes with SHANK1, essential for the structural and functional integrity of dendritic spines (By similarity).
• It negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway, acting as a competitive inhibitor of NFAT binding by calcineurin/PPP3CA. Related references: PMID:18218901
• Subcellularly, HOMER1 localizes to the cytoplasm, postsynaptic density, synapse, and dendritic spines.
• Post-translational modifications include acetylation and phosphorylation, and the protein features alternative splicing and a coiled coil domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region of HOMER1 (aa 125-354) and is expected to recognize all isoforms sharing this sequence; confirm isoform specificity if needed.
• For Western blot, a band around 40kDa is anticipated; validate in your sample system to account for tissue-specific expression and potential isoforms.
• In immunofluorescence (IF-P), consider co-staining with synaptic markers to confirm dendritic spine localization.
• For ELISA, optimize coating antigen and detection conditions using the purified recombinant protein if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted sourcing partner for diagnostic manufacturers and research institutes, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like HOMER1 involved in synaptic signaling and plasticity, we offer comprehensive raw-material solutions to accelerate your IVD development from concept to clinic.