HNRNPM Rabbit mAb for WB, IP, IHC-P, IF/ICC, ELISA - P52272

Core Product Specifications and Parameters

Biological Background: HNRNPM Function and Localization

• Heterogeneous nuclear ribonucleoprotein M (HNRNPM), also known as CEAR, HNRPM, HTGR1, NAGR1, HNRPM4, and HNRNPM4, is a member of the hnRNP family of RNA-binding proteins involved in pre-mRNA processing.
• HNRNPM functions as a pre-mRNA binding protein in vivo and exhibits strong avidity for poly(G) and poly(U) RNA homopolymers in vitro, playing a crucial role in alternative and constitutive splicing.
• It acts as a receptor for carcinoembryonic antigen in Kupffer cells, initiating tyrosine phosphorylation signaling cascades that induce cytokines such as IL-1 alpha, IL-6, IL-10, and tumor necrosis factor alpha.
• The protein localizes primarily to the nucleoplasm and nucleolus, consistent with its role in mRNA processing and ribonucleoprotein complex assembly.
• Post-translational modifications include acetylation, methylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its activity and protein-protein interactions.
• HNRNPM is a component of the spliceosome and is classified under mRNA processing and splicing keywords, highlighting its essential role in gene expression regulation.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using the antibody at suitable dilutions and validate with appropriate positive and negative controls, as the target protein has a predicted weight of approximately 78 kDa.
• The antibody has been tested in immunoprecipitation (IP) and can be used to enrich HNRNPM complexes; inclusion of RNase may help confirm RNA-dependent interactions.
• When performing immunofluorescence/ICC, nuclear and nucleolar staining patterns are expected; fixation and permeabilization conditions may need optimization.
• For IHC-P, antigen retrieval and tissue-specific expression patterns should be assessed; the antibody cross-reacts with human, mouse, and rat samples.
• ELISA applications can be developed with this antibody, but optimal coating and detection conditions must be determined experimentally.

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