HNRNPF Rabbit Polyclonal Antibody for WB, ELISA - P52597

Core Product Specifications and Parameters

Biological Background: HNRNPF Function and Localization

• HNRNPF (Heterogeneous nuclear ribonucleoprotein F), also designated nucleolin-like protein mcs94-1, is a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complexes.
• It plays a central role in pre-mRNA processing, providing the substrate for events that convert pre-mRNAs into functional, translatable mRNAs in the cytoplasm.
• HNRNPF specifically binds G-rich sequences in target pre-mRNAs and maintains the RNA in an unfolded state, facilitating subsequent processing steps.
• It participates in the regulation of alternative splicing, influencing the selection of splice sites during mRNA maturation.
• Subcellularly, HNRNPF localizes to the nucleus, predominantly in the nucleoplasm.
• It is expressed ubiquitously across human tissues, consistent with its fundamental role in mRNA biogenesis.
• The protein undergoes multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), as supported by proteomics data.
• Functional annotations include mRNA processing, mRNA splicing, ribonucleoprotein complex assembly, and spliceosomal components.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 315–415) of human HNRNPF; this region shows conservation between human and mouse, consistent with observed cross-reactivity.
• For Western blot, the predicted molecular weight is approximately 46 kDa; include appropriate loading controls and verify band size in your experimental system.
• For ELISA, consider using recombinant HNRNPF protein as a positive control to establish assay conditions.
• HNRNPF is a nuclear protein; ensure efficient nuclear extraction or total cell lysate preparation for reliable detection.
• Titrate the antibody to determine optimal working concentrations for WB and ELISA in your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies such as this anti-HNRNPF antibody. Our supply capabilities extend to antibody pairs, bulk ancillary reagents, and rare target sourcing, supporting projects in RNA biology and alternative splicing. With end-to-end services from concept to clinic, CamelBio bridges the gap between your research needs and reliable raw-material procurement.