HLA-DRB1 Rabbit Polyclonal Antibody for WB and ELISA - P01911 / P01912 / Q29974

Core Product Specifications and Parameters

Biological Background: HLA-DRB1 Function and Localization

• HLA-DRB1 encodes the beta chain of major histocompatibility complex class II (MHCII) molecules. In complex with the alpha chain HLA-DRA, it presents extracellular peptide antigens (10-30 amino acids) to CD4+ T cells, orchestrating adaptive immune responses against pathogens and tumors.
  Related references: PMID:8145819, PMID:15265931, PMID:16148104, PMID:22327072, PMID:31495665

• The protein is highly polymorphic, with numerous allelic variants (e.g., DRB1*01:01, *03:01, *04:01) that influence the peptide repertoire displayed to T cells and are associated with immune responsiveness and autoimmune diseases.

• Subcellular localization includes the cell membrane, endoplasmic reticulum, endosomes, lysosomes, and autolysosomes, reflecting its dynamic role in antigen processing, loading, and presentation.

• Tissue expression is restricted to professional antigen-presenting cells: monocytes/macrophages, dendritic cells, B cells, and thymic epithelial cells, as confirmed at the protein level.
  Related references: PMID:19830726, PMID:23783831, PMID:31495665

• Beyond antigen presentation, HLA-DRB1 also serves as a receptor for Epstein-Barr virus on lymphocytes, facilitating viral entry.

• Key post-translational modifications include glycosylation, disulfide bond formation, and ubiquitination (isopeptide bond), which are essential for MHCII stability and function.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is ~30 kDa. Consider using human or mouse spleen lysate as a positive control, given the tissue-specific expression in professional APCs.
• In ELISA, the antibody may be employed for both direct and indirect detection formats. Optimal coating concentrations and blocking conditions should be experimentally determined for each assay setup.
• Cross-reactivity with mouse makes this antibody suitable for studies in mouse models of antigen presentation, but confirm species-specific expression patterns in your target tissue.
• The immunogen spans the extracellular domain (aa 30-230), raising the possibility of recognition of both native and denatured HLA-DRB1; however, empirical validation under the intended experimental conditions is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw-material supply, including validated antibody pairs, optimized polyclonal antibodies like this anti-HLA-DRB1, and bulk ancillary reagents. Whether your focus is MHC class II antigen presentation, T cell immunology, or autoimmune disease biomarker development, CamelBio bridges the gap from concept to clinic with reliable sourcing and technical support. Let us streamline your procurement of rare-target raw materials and accelerate your assay development.