HLA-DPB1 Rabbit pAb for WB, IHC-P, ELISA - P04440

Core Product Specifications and Parameters

Biological Background: HLA-DPB1 Function and Localization

• HLA-DPB1 encodes the beta chain of the MHC class II molecule HLA-DP, a cell surface receptor essential for adaptive immune responses.
• It presents peptides derived from exogenous antigens that have been internalized via endocytosis or autophagy to CD4+ T helper cells, triggering immune activation.
• The peptide-binding cleft accommodates peptides of 10–30 residues, generated mainly by lysosomal proteolysis in antigen-presenting cells (APCs).
• Expressed primarily on professional APCs such as B cells, dendritic cells, and macrophages, as well as on gastrointestinal epithelial cells.
• Intracellularly, HLA-DPB1 traverses the endoplasmic reticulum, Golgi, and endosomal/lysosomal compartments before reaching the cell membrane.
• The protein undergoes glycosylation and contains a disulfide bond, and its stable surface expression depends on interaction with accessory molecules CD74 and HLA-DM during peptide loading.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 30-225) of human HLA-DPB1, suggesting potential reactivity against both native and denatured protein forms.
• For Western blot, consider using lysates from human B-cell lines or spleen tissue to detect the ~29 kDa band; validate in your system with appropriate controls.
• For IHC-P, human lymphoid organs (tonsil, lymph node) are suitable positive controls; optimize antigen retrieval and blocking conditions.
• Evaluate cross-reactivity in mouse and rat samples, as sequence homology may affect recognition; confirm with parallel human controls.
• ELISA performance may depend on coating conditions and antibody pair compatibility; empirical testing is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research labs, offering this HLA-DPB1 polyclonal antibody alongside a broad inventory of immunology-related reagents. Whether you need validated antibody pairs, optimized mono- or polyclonal antibodies, bulk ancillary reagents, or customized sourcing for rare targets like MHC class II proteins, CamelBio serves as your integrated supply partner from discovery to clinical application.