Histone H1t Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q07133

Core Product Specifications and Parameters

Biological Background: Histone H1t Function and Localization

• Histone H1t is a testis-specific linker histone that replaces somatic H1 subtypes during spermatogenesis, leading to a less compacted chromatin structure essential for meiotic chromosome dynamics.
• By forming relaxed chromatin, H1t facilitates homologous recombination and proper chromosome segregation during meiosis, critical for male fertility.
• H1t belongs to the histone H1 family, which binds nucleosomes and folds polynucleosome arrays into higher-order chromatin fibers; its variant tail domains confer unique biophysical properties.
• The protein localizes exclusively to the nucleus and associates with chromosomes, consistent with its role in chromatin architecture.
• Post‑translational modifications include acetylation, citrullination, and phosphorylation, which likely modulate its chromatin‑binding affinity and function during development.
• Expression is strictly testis‑specific and developmentally regulated, appearing in pachytene spermatocytes and persisting until late spermatid stages.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide from amino acids 110–209 of mouse H1t; this region lies in the C‑terminal tail domain, which is divergent among H1 subtypes and should confer good specificity.
• For western blot (WB), a band around 22 kDa is expected; validate loading controls and consider the possibility of multiple modified forms.
• In immunofluorescence on paraffin sections (IF‑P), confirm nuclear localization in testicular tissue; optimise antigen retrieval and antibody dilution for your specific protocol.
• The antibody has reported cross‑reactivity with mouse and rat; test cross‑species reactivity if using samples from other organisms.
• ELISA can be used for peptide‑based titering or screening; a direct or indirect format should be optimised with your assay buffer and detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This Histone H1t antibody serves as a reliable tool for developing assays targeting chromatin remodeling, meiosis, and male fertility biomarkers. Through our global network, we also handle rare-target raw‑material sourcing, helping you accelerate assay development from concept to clinic.