HFE2 Rabbit pAb - Q6ZVN8

Core Product Specifications and Parameters

Biological Background: HFE2 Function and Localization

• HFE2 (hemojuvelin) acts as a bone morphogenetic protein (BMP) coreceptor, enhancing BMP signaling to upregulate hepcidin (HAMP) expression, a key regulator of systemic iron homeostasis. Related references: PMID:18976966
• The protein is also known as RGMC (RGM domain family member C) and is critical for iron balance, with loss-of-function mutations causing juvenile hemochromatosis.
• Encoded by the HJV gene, multiple isoforms are produced via alternative splicing; the canonical isoform undergoes autocatalytic cleavage and is anchored to the cell membrane via a GPI moiety.
• Subcellular localization is primarily at the cell membrane, consistent with its role as a co-receptor.
• Tissue expression is highest in adult and fetal liver, heart, and skeletal muscle.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation, as indicated by UniProt keywords.
• The protein has a predicted molecular weight of ~45 kDa and contains an N-terminal signal peptide and a C-terminal GPI-anchor signal.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular domain (aa 1-174), which may be suitable for detecting both full-length and shed forms; consider testing lysates from HFE2-expressing tissues such as liver or skeletal muscle as positive controls.
• For Western blot, a band around 45 kDa is expected under reducing conditions; additional bands from cleavage products or isoforms may appear and should be validated using known cell lines or recombinant protein.
• When setting up ELISA, pairing with a detection antibody against a non-overlapping epitope is recommended; cross-reactivity with mouse and rat allows multi-species sample testing but requires individual assay optimization.
• Validate specificity and sensitivity in your own sample system; inclusion of appropriate controls (e.g., knockdown or knockout lysates) strengthens data interpretation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive range of IVD raw materials and services, from concept to clinical application. For studies on iron homeostasis and BMP signaling, we supply validated HFE2 antibodies, recombinant proteins, and ancillary reagents in bulk. Our sourcing capabilities extend to rare targets and custom development, ensuring reliable access to the critical raw materials needed for your hematology and metabolic disease assays.