HEXA Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P06865

Core Product Specifications and Parameters

Biological Background: HEXA Function and Localization

• HEXA encodes the alpha subunit of beta-hexosaminidase, also known as beta-N-acetylhexosaminidase subunit alpha. It forms the isozymes hexosaminidase A (αβ heterodimer) and S (αα homodimer).
• The enzyme hydrolyzes the non-reducing end N-acetyl-D-hexosamine and sulfated N-acetyl-D-hexosamine from glycoconjugates, including oligosaccharide chains, neutral glycolipids, and mucopolysaccharides.
  Related references:
  PMID:11707436
  PMID:16698036
  PMID:8123671
• Isozymes S and A are equally active on anionic bis-sulfated glycans, chondroitin-6-sulfate trisaccharide, and dermatan sulfate pentasaccharide, while isozyme B does not hydrolyze these substrates.
  Related references:
  PMID:11707436
• Only isozyme A is responsible for the degradation of GM2 gangliosides in the presence of the GM2A activator protein.
  Related references:
  PMID:8123671
  PMID:8672428
  PMID:9694901
• The protein is localized to the lysosome and plays critical roles in glycoconjugate and lipid metabolism.
• Mutations in HEXA lead to Tay-Sachs disease, a severe neurodegenerative gangliosidosis characterized by GM2 accumulation.
• Alternative splicing generates multiple transcript variants; the protein undergoes post-translational modifications including glycosylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 270–529 of human HEXA, covering a large C-terminal region. This design likely recognizes both full-length HEXA and potential alternative isoforms; verify specificity in your sample system.
• For Western blot, a band near 61 kDa is expected. Use appropriate loading controls and consider reducing conditions to resolve disulfide-linked complexes. Human liver or brain lysates can serve as positive controls.
• For IHC-P and IF-P, standard fixation with 4% PFA and antigen retrieval methods (e.g., citrate buffer, pH 6.0) are suggested. Cross-reactivity with mouse and rat tissues enables preclinical model analysis.
• For ELISA, optimize coating conditions using recombinant HEXA protein. A matched pair with a capture antibody targeting a non-overlapping epitope is recommended for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete one-stop sourcing platform for IVD raw materials, technical services, and expert consulting—guiding your project from concept to clinic. Our HEXA rabbit polyclonal antibody represents just one component of a broad portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and custom rare-target sourcing. We support researchers and diagnostic developers working on lysosomal storage diseases, lipid metabolism, and beyond with reliable supply-chain solutions.