HES5 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q5TA89

Core Product Specifications and Parameters

Biological Background: HES-5 Function and Localization

• HES-5 (Hairy and enhancer of split 5), also known as bHLHb38, is a member of the basic helix-loop-helix (bHLH) family of transcription factors. It acts as a transcriptional repressor of genes that require bHLH proteins for their transcription.
• HES-5 functions as a negative regulator of neurogenesis, playing a crucial role in maintaining neural progenitor cells and inhibiting premature neuronal differentiation.
• Consistent with its role in transcription regulation, HES-5 localizes to the nucleus where it binds DNA and exerts its repressive activity.
• Tissue-specific expression studies show HES-5 in fetal heart and brain tumors, suggesting roles in cardiac development and oncogenic processes.
• As a member of the HES family, HES-5 contains the conserved bHLH domain and a C-terminal WRPW motif for recruitment of co-repressors, key features for its repressive function.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 12-142 of human HES-5, covering the bHLH domain. This region is highly conserved, supporting observed cross-reactivity with mouse and rat.
• For Western blot (WB), HES-5 has a predicted molecular weight of ~18 kDa; ensure adequate gel resolution and use appropriate loading controls.
• In immunohistochemistry (IHC-P), validate antibody specificity in target tissues known to express HES-5 (e.g., fetal heart or brain tumor sections) with proper negative controls.
• For ELISA, consider pairing with a capture antibody if developing a sandwich assay, and optimize coating conditions based on recombinant HES-5 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a seamless procurement experience for high-quality IVD raw materials. Whether you need validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, or assistance sourcing rare targets like HES-5 for neurogenesis studies, our one-stop supply chain covers every stage from concept to clinic.