Heme Oxygenase 1 (HO-1/HMOX1) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P09601

Core Product Specifications and Parameters

Biological Background: Heme Oxygenase 1 Function and Localization

• Catalyzes the oxidative cleavage of heme to biliverdin, carbon monoxide, and ferrous iron, playing a key role in heme catabolism and iron recycling. Related references: PMID:11121422 PMID:19556236 PMID:7703255

• Protects against programmed cell death by preventing free heme from sensitizing cells to apoptosis, demonstrating a cytoprotective function. Related references: PMID:20055707

• During SARS-CoV-2 infection, promotes viral ORF3A-mediated autophagy, though not required for ORF3A-induced reticulophagy.

• Localizes to the endoplasmic reticulum membrane as a transmembrane protein.

• Expressed at higher levels in renal cancer tissue compared to normal tissue (at protein level).

• Also known as HSP32; belongs to the heme oxygenase family and is classified under keywords including endoplasmic reticulum, heme, iron, metal-binding, oxidoreductase, phosphoprotein, apoptosis, and host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–265) of human HMOX1, which is well-conserved across human, mouse, and rat orthologs, supporting the observed cross-reactivity.

• For Western blotting, the expected molecular weight is approximately 33 kDa; note that post-translational modifications or protein dimerization may affect migration.

• Consider validating antibody specificity using appropriate controls (e.g., knockdown or knockout) in your sample system, especially for immunolocalization studies.

• For immunofluorescence and immunohistochemistry, optimize fixation and permeabilization conditions to detect ER membrane-localized HO-1.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, technical services, and consulting for diagnostic manufacturers and research laboratories. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing, supporting workflows from concept to clinic. The Heme Oxygenase 1 (HO-1/HMOX1) rabbit polyclonal antibody caters to studies in heme metabolism, oxidative stress, cancer biology, and viral–host interactions, backed by our comprehensive supply chain expertise.