HDAC9 Rabbit mAb - Q9UKV0

Core Product Specifications and Parameters

Biological Background: HDAC9 Function and Localization

• HDAC9 is a histone deacetylase that catalyzes the removal of acetyl groups from lysine residues on histones H2A, H2B, H3, and H4, leading to transcriptional repression and epigenetic regulation.
• It represses MEF2-dependent transcription, playing critical roles in cell cycle progression, developmental events, and skeletal myogenesis inhibition.
• Isoform 3 lacks catalytic active site residues and is enzymatically inactive; it represses transcription by recruiting HDAC1 and/or HDAC3.
• HDAC9 protects neurons from apoptosis by inhibiting JUN phosphorylation via MAPK10 and repressing JUN transcription through HDAC1 recruitment.
• Located primarily in the nucleus, HDAC9 is broadly expressed, with highest levels in brain, heart, muscle, and testis. Isoform 3 is detected in bladder carcinoma cells.
• The protein is subject to alternative splicing, phosphorylation, and ubiquitination, and contains a zinc-binding domain essential for its hydrolase activity.

Experimental Guidance and Technical Tips

• Because the immunogen is located within the N-terminal region (amino acids 1–100) of human HDAC9, the antibody may recognize multiple isoforms sharing this sequence. Verify isoform specificity in the relevant experimental system.
• For Western blot, a band around 111 kDa is expected for the full-length protein; consider using appropriate loading controls and validate detection in human and rat samples.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and antibody concentration, and confirm staining in positive control tissues such as brain or heart.
• In ELISA applications, consider pairing with a detection antibody against a non-overlapping epitope if designing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinic. The HDAC9 Rabbit mAb is part of our extensive portfolio for epigenetic and transcriptional regulation research, enabling reliable detection in multiple applications. For customized sourcing of rare targets or large-scale production, our team provides tailored solutions to meet your project requirements.