HDAC8 Rabbit mAb for IHC-P, IF/ICC, IP, ELISA - Q9BY41

Core Product Specifications and Parameters

Biological Background: HDAC8 Function and Localization

• Histone deacetylase 8 (HDAC8), also known as HDACL1, is a member of the class I histone deacetylase family that catalyzes the removal of acetyl groups from lysine residues on the N‑terminal tails of core histones (H2A, H2B, H3, H4), leading to chromatin compaction and transcriptional repression. This activity is central to epigenetic regulation of gene expression, cell cycle progression, and developmental processes. Related references: PMID:10748112 • PMID:10922473 • PMID:10926844
• HDAC8 functions within large multiprotein complexes to exert its deacetylase activity, and additionally deacetylates the cohesin subunit SMC3, thereby regulating the release of cohesin complexes from chromatin during mitosis. Related references: PMID:22885700
• Beyond its deacetylase function, HDAC8 possesses protein decrotonylase activity, catalyzing the removal of crotonyl groups ((2E)-butenoyl) from histones, expanding its role in epigenetic modification. Related references: PMID:28497810
• The protein may contribute to smooth muscle cell contractility, indicating a non‑histone regulatory role. Related references: PMID:15772115
• HDAC8 localizes predominantly to the nucleus and chromosomes, but can also be detected in the cytoplasm, suggesting dynamic subcellular shuttling.
• Tissue expression is widespread but at low levels; higher expression is observed in heart, brain, kidney, pancreas, liver, lung, placenta, and prostate.
• The protein is subject to phosphorylation and alternative splicing, and mutations in HDAC8 are associated with diseases including Cornelia de Lange syndrome and intellectual disability, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen (a synthetic peptide encompassing amino acids 1–100 of human HDAC8) targets the N‑terminal region; consider verifying antibody specificity by peptide blocking or knockdown experiments.
• For IHC‑P, perform antigen retrieval using heat‑mediated methods (e.g., citrate buffer, pH 6.0) and test with tissues known to express HDAC8, such as heart or brain.
• In IF/ICC, ensure appropriate permeabilization (e.g., 0.1% Triton X‑100) and use a nuclear counterstain to confirm expected nuclear localization.
• For IP, optimize antibody‑to‑lysate ratios and validate co‑immunoprecipitated partners in your cell line of interest.
• ELISA may require careful titration of both capture and detection antibodies; include a negative control well to assess background.
• Cross‑reactivity with mouse and rat is predicted based on sequence homology, but independent validation in these species is advisable before large‑scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom procurement for rare targets. With this HDAC8 Rabbit mAb, researchers gain a reliable tool for epigenetics and chromatin regulation studies, backed by CamelBio’s commitment to quality, consistency, and technical support from concept to clinic.