HDAC6 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q9Z2V5

Core Product Specifications and Parameters

Biological Background: HDAC6 Function and Localization

• HDAC6 (histone deacetylase 6, also designated mHDA2) is a cytoplasmic protein deacetylase that acts on a broad range of non-histone substrates, including α-tubulin, CTTN, SQSTM1, and transcription factors, thereby influencing diverse cellular pathways. Related references: PMID:12606581, PMID:19893491, PMID:26746851

• It is a central regulator of microtubule dynamics through deacetylation of α-tubulin, which destabilizes dynamic microtubules and is required for cilia disassembly; this function underpins its roles in cell motility and intracellular transport. Related references: PMID:12486003, PMID:12606581, PMID:19893491, PMID:27737934

• HDAC6 serves as a critical adapter in the aggresome pathway: it recognizes polyubiquitinated misfolded proteins and directs them to the perinuclear aggresome, facilitating their clearance by autophagy. Related references: PMID:22819792

• The enzyme modulates antiviral innate immunity by deacetylating the viral RNA sensor RIGI, an essential step for downstream signaling; it also promotes autophagy completion via deacetylation of CTTN. Related references: PMID:26746851

• HDAC6 contributes to developmental and differentiation processes: it deacetylates PRDM16 and promotes odontoblast differentiation by repressing RUNX2 expression. Related references: PMID:35075301, PMID:35922041

• Subcellularly, HDAC6 localizes predominantly to the cytoplasm and cytoskeleton (including microtubules, centrosomes, and basal bodies), but also appears in the nucleus and in cell projections such as axons, dendrites, and cilia.

• The protein is expressed in specific neural populations (cortex neurons and Purkinje cells) and in keratinocytes. HDAC6 undergoes multiple post-translational modifications (phosphorylation, methylation, ubiquitination) and contains zinc-finger domains, reflecting its classification as a metal-binding chromatin regulator with transferase and hydrolase activities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 836–1037) of mouse HDAC6 encompassing the second deacetylase domain and a ubiquitin‑binding zinc finger; sequence homology supports cross‑reactivity with human and rat proteins.
• For Western blot, consider an initial dilution range of 1:500–1:2000 and validate in lysates from cells or tissues known to express HDAC6 (expected molecular weight ~126 kDa).
• In immunofluorescence and immunohistochemistry, HDAC6 localizes to cytoplasmic microtubule networks; include an appropriate permeabilization step and co‑staining with cytoskeletal markers where relevant.
• Because HDAC6 participates in aggresome formation and autophagy, double‑labeling with ubiquitin or proteasome components can provide functional context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, connecting concept to clinic with a full spectrum of antibodies, protein reagents, and technical support. Our HDAC6 antibody portfolio serves autophagy, chromatin, and cytoskeleton research, complementing validated monoclonal/polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents. Whether you need standard catalog items or custom rare‑target sourcing, we streamline procurement to accelerate your diagnostic development.