HDAC5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UQL6

Core Product Specifications and Parameters

Biological Background: HDAC5 Function and Localization

• HDAC5 (histone deacetylase 5) catalyzes the removal of acetyl groups from lysine residues on core histones H2A, H2B, H3, and H4, promoting chromatin condensation and transcriptional silencing. Related references: PMID:10220385
• It functions within large multiprotein complexes to regulate gene expression, influencing cell cycle progression, cellular differentiation, and developmental processes.
• HDAC5 is a member of the class II histone deacetylase family; alternative splicing generates multiple isoforms with potentially distinct regulatory roles.
• The protein dynamically shuttles between the nucleus and cytoplasm, a process critical for muscle differentiation where its nuclear export allows expression of myocyte enhancer factors like MEF2C.
• HDAC5 acts as a corepressor of retinoic acid receptor alpha (RARA), mediating its deacetylation and contributing to the repression of microRNA-10a, thereby modulating the inflammatory response. Related references: PMID:28167758
• Post-translational modifications, including phosphorylation, acetylation, and ubiquitin-like modifications (e.g., SUMOylation), regulate HDAC5 activity, subcellular localization, and protein stability.
• HDAC5 is widely expressed across human tissues, with detection in heart, brain, kidney, pancreas, liver, skeletal muscle, and placenta. Related references: PMID:10220385

Experimental Guidance and Technical Tips

• The immunogen was derived from a peptide within amino acids 350–450 of human HDAC5; this region is predicted to be specific and may help discriminate HDAC5 from other class II HDACs.
• For western blotting, note the predicted molecular weight of ~122 kDa; consider using appropriate gel percentage and transfer conditions optimized for high‑molecular‑weight proteins.
• In immunofluorescence/ICC experiments, HDAC5 can localize to either the nucleus or cytoplasm depending on cellular context and stimuli; validate the staining pattern in the target cell system with proper controls.
• For ELISA‑based detection, preliminary titration and optimal coating conditions are recommended when using the antibody to capture or detect HDAC5.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research institutes, and laboratories a streamlined one‑stop access to high‑quality IVD raw materials, technical services, and consulting—from concept through clinical application. With this HDAC5 rabbit polyclonal antibody, we support your epigenetic, cancer, and muscle development research programs. Our supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing of rare‑target raw materials, ensuring you find the right reagents for your assay development.